灵长类自体胚胎干细胞成功构建

Core Insights - The research team from Kunming University of Science and Technology successfully developed healthy live monkeys and genetically matched autologous embryonic stem cells from a single primate embryo using embryo splitting technology, marking a significant advancement in regenerative medicine [1][2] - The study published in the journal Nature Communications highlights the importance of obtaining genetically matched embryonic stem cells to address issues of immune rejection and ethical concerns associated with traditional stem cell therapies [1] Summary by Categories Research Methodology - The team optimized embryo splitting techniques by simulating the natural formation of identical twins, performing splits at the 4-cell and 8-cell stages, with a more efficient 3:5 strategy at the 8-cell stage [1] - From 23 pairs of split embryos, two healthy monkeys and autologous stem cell lines were successfully generated, with one case resulting in a successful cell line establishment before mid-pregnancy miscarriage [1] Findings and Comparisons - Single-cell transcriptome analysis revealed that autologous embryonic stem cells exhibited lower cell heterogeneity, reduced transcriptional noise, and more significant expression of genes related to genomic stability compared to induced pluripotent stem cells and somatic cell nuclear transfer embryonic stem cells [2] - Under feeder layer culture conditions, induced pluripotent stem cells showed a stronger differentiation trend, while autologous embryonic stem cells were more similar to somatic cell nuclear transfer embryonic stem cells [2] Clinical Implications - The establishment of autologous embryonic stem cells from split embryos presents a new method for generating pluripotent stem cells and provides an ideal model for assessing the functional differences of various stem cell types in vivo [2] - The research has potential clinical applications in organ repair and disease treatment, laying a crucial foundation for future regenerative medicine applications [2]