INVESTOR ALERT: Class Action Lawsuit Filed on Behalf of Alto Neuroscience, Inc. (ANRO) Investors – Holzer & Holzer, LLC Encourages Investors With Significant Losses to Contact the Firm

Group 1 - A shareholder class action lawsuit has been filed against Alto Neuroscience, Inc. alleging that the company made materially false and/or misleading statements regarding its business and operations [1] - The lawsuit claims that ALTO-100 was less effective in treating Major Depressive Disorder (MDD) than previously communicated to investors, leading to overstated clinical, regulatory, and commercial prospects [1] - As a result of these allegations, the lawsuit asserts that Alto's overall business and financial prospects were also overstated [1] Group 2 - Investors who purchased shares of Alto between February 2, 2024, and October 22, 2024, and suffered significant losses are encouraged to discuss their legal rights [2] - The deadline for investors to request to be appointed as lead plaintiff in the case is September 19, 2025 [3] - Holzer & Holzer, LLC is a law firm specializing in representing shareholders and has a history of recovering significant amounts for investors affected by corporate misconduct [3]