人造细胞内核苷酸从头合成代谢通路构建

Core Insights - The research team at Harbin Institute of Technology has successfully constructed a de novo nucleotide synthesis metabolic pathway within artificial cells, laying the foundation for autonomous artificial cell development [1][2] - Nucleotides are essential components for RNA synthesis, and the ability to synthesize them in situ is crucial for the autonomy and long-term stability of artificial cells [1] Group 1 - The metabolic pathway starts with ammonium bicarbonate to synthesize uridine triphosphate (UTP) and involves eight enzymes, including CPS, ATC, DHO, DHODH, RPPK, UMPs, UK, and NDK [2] - An ATP regeneration system composed of creatine kinase (CK) and phosphocreatine was introduced to drive the metabolic pathway [2] - Under optimized conditions, the pathway can produce 0.85 millimoles per liter of UTP within three hours, enabling RNA transcription in artificial cells [2]