奶牛多组织单细胞表达图谱问世

Core Insights - The research team at China Agricultural University has developed the world's first multi-tissue single-cell expression atlas for dairy cows, covering 59 tissues and 1.79 million cells, which is crucial for understanding genetic regulation mechanisms of important traits in cattle and advancing precision breeding [1][2] Group 1: Research Significance - This study fills a gap in the field of single-cell biology for dairy cows, providing essential foundational data for genetic breeding, immunology research, and comparative medicine [2] - The technical approach and analytical framework established in this research can serve as a reference for constructing single-cell atlases for other livestock, promoting innovation in breeding technologies and ensuring the utilization of agricultural animal genetic resources [2] Group 2: Methodology and Findings - The research utilized single-cell transcriptome sequencing technology on one Holstein cow fetus, four calves, and ten adult Holstein cows (including both bulls and cows), resulting in the annotation of 131 cell types across seven major cell lineages [1] - The study successfully identified high-quality cells from 59 tissues, which include immune cells, endothelial cells, epithelial cells, stromal cells, nerve cells, muscle cells, and reproductive cells [1]