此物一出，万年不降解！最新成果发现3种PFAS已广泛存在于血液

Core Viewpoint - The research conducted by the team led by Cai Yaqi from the Chinese Academy of Sciences establishes a QuEChERS-UPLC-MS/MS method for detecting 20 types of PFAS in human blood, revealing a 100% detection rate for 10 types of PFAS in 15 samples from Shandong Province, with PFOA, PFOS, and 6:2 Cl-PFESA identified as major pollutants [2][3][4]. Summary by Sections Abstract - The study emphasizes the importance of measuring PFAS concentrations in human blood to understand exposure levels and assess health risks. A QuEChERS-UPLC-MS/MS method was developed, utilizing pure acetonitrile for extraction and achieving a total PFAS concentration range of 7.65-51.3 ng·mL⁻¹ in the analyzed samples [4][24]. Experimental Objectives - The objectives include establishing a simple and efficient QuEChERS method for extracting and purifying PFAS from human blood, optimizing UPLC-MS/MS parameters for high sensitivity and accurate quantification, and validating the method's reliability through various metrics [5][6][7]. Experimental Principles - PFAS are hydrophobic and chemically stable, leading to accumulation in blood. The QuEChERS method employs acetonitrile extraction, salt-induced phase separation, and solid-phase extraction to remove matrix interferences, while UPLC-MS/MS provides qualitative and quantitative analysis [8]. Instruments and Reagents - The instruments used include a UPLC system and a triple quadrupole mass spectrometer, along with various auxiliary devices. The target compounds consist of 20 types of PFAS, with isotopically labeled standards and specific solvents and chemicals utilized for the analysis [9][10]. Experimental Methods - Blood samples were collected from 15 volunteers in Shandong, processed using the QuEChERS method, and analyzed using UPLC-MS/MS under optimized conditions [11][12][15]. Method Validation - The method demonstrated a linear range for 20 PFAS from 0.05 to 50 ng·mL⁻¹, with detection limits between 0.010-0.063 ng·mL⁻¹ and quantification limits from 0.032-0.210 ng·mL⁻¹. Recovery rates were satisfactory, meeting quantitative analysis requirements [21][22][23]. Actual Sample Analysis - Analysis of the 15 blood samples showed a total PFAS concentration range of 7.65-51.3 ng·mL⁻¹, with a 100% detection rate for 10 PFAS, highlighting PFOA, PFOS, and 6:2 Cl-PFESA as significant pollutants [23]. Conclusion - The established QuEChERS-UPLC-MS/MS method is efficient and reliable for accurately measuring PFAS in blood, indicating widespread presence of traditional and alternative PFAS in human blood, which raises health risk concerns [24].