建立高效液相色谱法测定从化乌橄榄中6种酚类物质的分析方法

Core Viewpoint - The article establishes an analytical method using high-performance liquid chromatography (HPLC) to determine six phenolic substances in Conghua olives, optimizing various conditions for effective separation and detection [2][4][40]. Methodology - The method involves weighing 0.5g of dried Conghua olive powder, dissolving it in isopropyl alcohol, and subjecting it to ultrasonic extraction for 30 minutes, followed by solid-phase extraction and filtration before HPLC analysis [4][6]. - The Eclipse XDB-C18 chromatographic column was selected for separation, and a diode array detector was used to optimize the testing conditions [4][12]. Results - The study found that the peak areas of gallic acid, hydroxytyrosol, oleuropein, and elenolic acid showed good linear relationships within specified concentration ranges, with R² values greater than 0.995 [4][5]. - Detection limits for the six substances were established, with gallic acid at 2.09 mg/kg and hydroxytyrosol at 2.20 mg/kg, among others [5][33]. Recovery and Precision - The recovery rates for the substances ranged from 84% to 116%, with relative standard deviations between 0.014% and 0.15%, indicating the method's reliability [5][34]. Conclusion - The optimized method provides a reliable approach for determining the content of six phenolic substances in Conghua olives, contributing valuable insights for researchers in the field [40].