6种复合分析保护剂在果蔬中农药残留检测中的应用

Core Viewpoint - The article evaluates the compensation efficiency of six composite analytical protectants in pesticide residue detection in fruits and vegetables using gas chromatography-tandem mass spectrometry (GC-MS/MS) technology, establishing a method for detecting 45 types of pesticide residues with improved recovery rates and sensitivity compared to existing standards [2][4]. Group 1: Methodology and Results - The study utilized an orthogonal test to optimize the formulation of composite protectants, analyzing their effects on matrix effects (ME) during pesticide residue detection [4][6]. - The ideal composite protectant combination, AP3, significantly improved chromatographic peak shapes and achieved comprehensive compensation effects, with a linear range of 2.5–200.0 µg/L and correlation coefficients (r²) greater than 0.995 [4][28]. - Detection limits for winter melon were established at 0.25–1.95 µg/kg, and for pear at 0.16–1.87 µg/kg, with quantification limits for winter melon at 0.91–5.12 µg/kg and for pear at 0.71–5.34 µg/kg [4][28]. Group 2: Analysis of Matrix Effects - The matrix effects were evaluated for 45 pesticide residues, revealing significant differences in ME for the same pesticide across different matrices, with some pesticides exhibiting strong ME in certain fruits [17][24]. - The study identified that the commonly used analytical protectants could vary in their effectiveness based on the type of pesticide and its concentration, necessitating a composite approach for optimal results [6][25]. Group 3: Composite Analytical Protectants - The six analytical protectants evaluated included L-gulonic acid-γ-lactone, D-sorbitol, shikimic acid, 3-ethoxy-1,2-propanediol, D-panthenol, and di(2-hydroxyethyl)iminotris(hydroxymethyl)methane, each contributing differently to ME compensation [4][24]. - The study concluded that while single protectants have limitations, a composite approach can effectively address the diverse nature of pesticide residues in complex matrices [25][28].