Core Insights - PacBio has introduced CiFi, a new method that enables chromosome-scale, haplotype-resolved genome assemblies from minimal input material through a single sequencing run [1][4] - CiFi integrates chromatin conformation capture (3C) with PacBio HiFi long-read sequencing, significantly enhancing the information content of proximity ligation experiments [1][5] Group 1: Methodology and Advantages - CiFi addresses limitations of traditional Hi-C methods by generating long, highly accurate reads that capture multiple chromatin interactions within a single molecule [2][5] - The method improves mapping in repetitive regions, enhances low input performance, enables multi-contact resolution, and simplifies project complexity through a single platform [3][4] - By using CiFi with Revio SPRQ chemistry, researchers can achieve reference-quality assemblies with fewer cells, libraries, and sequencing runs, thus lowering barriers for genome projects [4][6] Group 2: Demonstration and Results - In a demonstration, the CiFi method was applied to the prairie and meadow vole, resulting in uncurated assemblies with scaffold N50 values exceeding 100 million base pairs [6] - Telomeric sequences were detected at both ends of many scaffolds, showcasing the capability of CiFi to deliver chromosome-scale, reference-quality assemblies from just one sequencing run [6] Group 3: Company Background - PacBio is a leading life science technology company that develops advanced sequencing solutions aimed at resolving genetically complex problems across various research applications [8] - The company's products, including HiFi long-read sequencing, cater to fields such as human germline sequencing, plant and animal sciences, infectious disease, and oncology [8]