As filed with the Securities and Exchange Commission on July 25, 2025 Registration No. 333- UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM S-1 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 Actuate Therapeutics, Inc. (Exact name of registrant as specified in its charter) Delaware 8731 47-3044785 (State or other jurisdiction of incorporation or organization) (Primary Standard Industrial Classification Code Number) (I.R.S. Employer Identification Number) 1751 River Run, ...