UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended June 30, 2022 OR ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-39527 PRELUDE THERAPEUTICS INCORPORATED (Exact Name of Registrant as Specified in its Charter) (State or other jurisdiction of inc ...

Financial Performance - The net loss for the three months ended March 31, 2022, was $29.5 million, compared to a net loss of $21.3 million for the same period in 2021, representing an increase of $8.2 million [84]. - Total operating expenses for the three months ended March 31, 2022, were $30.3 million, up from $22.0 million in 2021, reflecting an increase of $8.3 million [96]. - The company has an accumulated deficit of $248.6 million as of March 31, 2022 [84]. - Cash used in operating activities was $21.9 million for the three months ended March 31, 2022, compared to $16.1 million for the same period in 2021 [106]. - General and administrative expenses rose by $2.0 million to $7.5 million for the three months ended March 31, 2022, from $5.5 million in the same period of 2021 [99]. - Other income, net increased by $0.2 million to $0.8 million for the three months ended March 31, 2022, primarily due to research and development tax credits [100]. Research and Development - Research and development expenses increased to $22.8 million for the three months ended March 31, 2022, from $16.5 million in 2021, an increase of $6.4 million [96]. - Research and development expenses increased by $6.4 million to $22.8 million for the three months ended March 31, 2022, compared to $16.5 million for the same period in 2021 [97]. - Research and development expenses by program included $5.4 million for discovery programs and $11.2 million for internal costs for the three months ended March 31, 2022 [98]. - PRT811, a candidate in the PRMT5 program, is being developed for biomarker-selected patients, focusing on its superior safety profile and brain penetrant properties [78]. - PRT1419, designed as a potent inhibitor of MCL1, is currently enrolling patients in Phase 1 clinical trials for hematologic malignancies [80]. Cash and Funding - As of March 31, 2022, the company had $266.2 million in cash, cash equivalents, and marketable securities, expected to fund operations into the second half of 2024 [86]. - The company expects existing cash resources to fund operating expenses into the second half of 2024 [101]. - The company plans to finance operations through equity sales, debt financings, or collaborations, highlighting the need for substantial additional capital [85]. - Net cash provided by investing activities was $41.5 million for the three months ended March 31, 2022, primarily from maturities of marketable securities [109]. - Financing activities provided $0.2 million from the exercise of stock options during the three months ended March 31, 2022, compared to $161.8 million in the same period of 2021 [110]. Revenue Generation - The company has not recognized any revenue to date and does not expect to generate revenue from product sales in the foreseeable future [88]. - The company has not yet commercialized any product and does not expect to generate revenue from product sales for several years [101]. - The company has advanced four clinical candidates and plans to file two INDs in the second half of 2022 [76].

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2021 OR ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 FOR THE TRANSITION PERIOD FROM TO Commission File Number 001-39527 PRELUDE THERAPEUTICS INCORPORATED (Exact name of Registrant as specified in its Charter) Delaware 81-1384762 (State or other ju ...

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended September 30, 2021 OR ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-39527 PRELUDE THERAPEUTICS INCORPORATED (Exact Name of Registrant as Specified in its Charter) | Delaware | 81-1384762 | | -- ...

UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended June 30, 2021 OR ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to (Exact Name of Registrant as Specified in its Charter) (State or other jurisdiction of incorporation or organization) 200 Powder Mill Road Wilmington, Delawar ...

| --- | --- | --- | --- | --- | --- | |---------------------------------------|-------|-------|-------|-------|-------| | | | | | | | | Prelude | | | | | | | THERAPEUTICS | | | | | | | ............. ............. ......... | | | | | | | | | | | | | | Corporate Presentation May 2021 | | | | | | Disclaimer This presentation contains "forward-looking" statements within the meaning of the "safe harbor" provisions of the Private Securities Litigation Reform Act of 1995, including, but not limited to: our plans t ...

FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended March 31, 2021 OR UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-39527 PRELUDE THERAPEUTICS INCORPORATED (Exact Name of Registrant as Specified in its Charter) (State or other jurisdiction of in ...

| --- | --- | --- | --- | --- | --- | |-----------------------------------|-------|-------|-------|-------|-------| | | | | | | | | Prelude | | | | | | | THERAPEUTICS | | | | | | | ............. .............. | | | | | | | | | | | | | | Corporate Presentation March 2021 | | | | | | Disclaimer This presentation contains "forward-looking" statements within the meaning of the "safe harbor" provisions of the Private Securities Litigation Reform Act of 1995, including, but not limited to: our plans to develop a ...

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2020 OR ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 FOR THE TRANSITION PERIOD FROM TO Commission File Number 001-39527 PRELUDE THERAPEUTICS INCORPORATED (Exact name of Registrant as specified in its Charter) Delaware 81-1384762 (State or other ju ...

☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-39527 UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, DC 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended September 30, 2020 OR PRELUDE THERAPEUTICS INCORPORATED (Exact Name of Registrant as Specified in its Charter) | Delaware | 81-1384762 | | -- ...