我国科学家研制出多色微型化双光子显微镜 对大脑进行“彩色直播”（探一线）

Core Viewpoint - A team of researchers from Peking University and Beijing Information Science and Technology University has developed a multi-color miniaturized two-photon microscope, enabling high-resolution imaging of the deep brain in freely moving mice, marking a significant advancement in brain science research [1][2]. Group 1: Technological Advancements - The new microscope allows for "color live broadcasting" of brain activity, significantly enhancing the understanding of neural circuits and the pathological mechanisms of brain diseases [1]. - The fourth-generation system achieves breakthroughs in multi-color excitation, deep brain imaging, and multi-scale observation, improving upon previous iterations that focused on single-color imaging and limited depth [1][2]. - The microscope's imaging depth exceeds 850 micrometers, three times deeper than previous miniaturized two-photon technologies, allowing for detailed observation of deeper brain structures [2]. Group 2: Research Applications - The technology enables the observation of complex interactions between different cell types using various fluorescent proteins, providing insights into cellular behavior in conditions like Alzheimer's disease [2]. - The microscope has already been successfully commercialized and exported to multiple countries, indicating its potential for widespread application in neuroscience research [3]. - Future research plans include integrating optogenetics to create a "read-write" loop for controlling specific neurons and increasing imaging speed to record brain activity in real-time [3].