Cell：吴军团队开发强制线粒体自噬技术，揭示线粒体在多能干细胞和胚胎发育中的关键作用

Core Insights - The article discusses the significant role of mitochondria in mammalian development and introduces a new method for enforced mitophagy that allows for the reduction or complete removal of mitochondria, revealing their influence on pluripotency and embryonic development [3][15]. Group 1: Research Findings - The study published by Professor Wu Jun's team at the University of Texas Southwestern Medical Center demonstrates that enforced mitophagy can lead to a reduction in mitochondrial quantity, which subsequently delays pre-implantation embryonic development in mice [3][11]. - The research indicates that pluripotent stem cells (PSCs) lacking mitochondria can survive for 3-5 days in vitro but cease to divide, suggesting that these cells can compensate for the absence of mitochondria by taking over energy production and other functions typically performed by mitochondria [8][13]. - The study also reveals that the enforced mitophagy method can be applied across different species and cell types, potentially opening new avenues for research and treatment of mitochondrial diseases [8][15]. Group 2: Methodology - The enforced mitophagy technique involves expressing the PRKN protein in cells, which promotes the degradation of dysfunctional mitochondria, followed by treatment with mitochondrial uncouplers to stimulate extensive mitophagy [6][8]. - The research team successfully generated PSCs devoid of mitochondria and assessed the gene expression changes, finding that 788 genes became less active while 1696 genes became more active, indicating a shift in cellular function [8][13]. - The study further explores the fusion of human PSCs with those from non-human primates, revealing that these hybrid cells selectively retain human mitochondrial DNA, demonstrating the interchangeability of mitochondrial support for pluripotency across species [9][13]. Group 3: Implications - The findings suggest that a significant reduction in mitochondrial content can hinder embryonic development, with a 65% loss leading to implantation failure and a 33% loss resulting in developmental delays [11][13]. - The research provides a powerful tool for investigating the roles of mitochondria in cellular functions, organ development, aging, and evolutionary biology, potentially impacting future therapeutic strategies for mitochondrial diseases [15].