华大×南方科技大学合作发表最新Cell论文：解码生命动态调控“时空密码”

Core Viewpoint - The research conducted by BGI and Southern University of Science and Technology has created a groundbreaking 3D single-cell spatiotemporal multi-omics atlas of Drosophila development, providing unprecedented insights into the molecular mechanisms of cell-type differentiation and developmental biology [2][4][25]. Group 1: Research Methodology - The research team utilized BGI's proprietary spatiotemporal omics technology, Stereo-seq, along with single-cell sequencing techniques (scRNA-seq and scATAC-seq) to sample key stages of Drosophila development, generating over 3.8 million spatially resolved single-cell transcriptomes [3][7]. - The Spateo algorithm was employed to reconstruct high-precision 3D models, allowing for detailed analysis of tissue morphology and gene expression dynamics [7]. Group 2: Key Findings - The study systematically analyzed the spatiotemporal dynamics of cell-type differentiation in Drosophila, revealing critical regulatory networks that govern developmental processes [4][9]. - A "differentiation trajectory map" was constructed, elucidating the molecular mechanisms behind cell fate determination, with transcription factors acting as key regulators [9][22]. - The research identified previously uncharacterized transcription factors that play significant roles in the nervous, digestive, and endocrine systems, expanding the understanding of developmental regulation [9][22]. Group 3: Developmental Patterns - The 3D multi-omics atlas revealed the spatial patterns of tissue differentiation, with distinct developmental modes observed in the fat body and fore/hind gut [11][13]. - The fat body exhibited a dispersed differentiation pattern, while the fore/hind gut showed a centralized characteristic, providing new evidence for understanding embryonic gut formation [13]. Group 4: Central Nervous System Development - The study highlighted key nodes in the morphological remodeling of the central nervous system, identifying new regulatory factors associated with neural progenitor cell migration [14][22]. - The dynamic changes in midgut cell types and their spatial organization were tracked, revealing that midgut stem cells had already "prepared" for future differentiation during the larval stage [18][19]. Group 5: Implications for Human Health - Drosophila serves as a crucial model organism in various biological fields, sharing approximately 70% of disease-related genes with humans, thus providing valuable insights into human developmental diseases [9][25]. - The findings regarding the regulation of copper cells in the midgut may offer new mechanisms for understanding organ development in humans [22].