浙江工业大学发表最新Science论文

Core Viewpoint - The research presents a novel method for enantioconvergent benzylic C(sp3)–N coupling using a copper-substituted nonheme enzyme, expanding the scope of non-natural biocatalysis with transition metals [2][3]. Group 1: Research Development - The study was conducted by teams from Zhejiang University, Johns Hopkins University, and Utah State University, and published in the journal Science [2]. - The research focuses on developing a biocatalytic system by substituting iron in non-heme iron enzymes with copper and optimizing surrounding amino acids to facilitate the reaction [5]. Group 2: Methodology and Findings - The team utilized Rhodamine B as a photoredox catalyst and identified a copper-substituted phenylalanine hydroxylase that promotes the decarboxylative amination reaction between N-hydroxybenzoyl amine and aniline [5]. - Directed evolution was employed to reshape the active site, resulting in high enantioselectivity for most substrates [5]. Group 3: Implications - This research cleverly combines the advantages of photochemistry and enzymatic catalysis, achieving free radical C–N coupling under mild conditions, which traditional metal enzymes cannot accomplish [6].