西湖大学开发从天然相分离机制到全能细胞工程化的新工具

Core Viewpoint - The article highlights the significance of transfection technology in life sciences and medical research, emphasizing the breakthrough of ProteanFect, a novel transfection product based on protein coacervates, which addresses the longstanding "transfection challenge" faced by researchers [1][2]. Group 1: Transfection Technology and Challenges - Transfection technology is crucial for exploring complex mechanisms of diseases and developing innovative therapies, yet researchers have struggled with traditional methods that have limitations such as low stability and safety concerns [1]. - Traditional methods like liposomes, viral vectors, and electroporation often lead to cell damage, particularly when working with precious samples like primary immune cells and neurons [1]. Group 2: Introduction of ProteanFect - ProteanFect is the world's first transfection product based on protein coacervates, designed to overcome the limitations of traditional methods by achieving high delivery efficiency with low cytotoxicity [1][2]. - Since its launch, ProteanFect has gained recognition as a comprehensive solution in the industry, validated by solid experimental data and positive user feedback [2]. Group 3: Mechanism and Functionality - The product utilizes the natural phenomenon of liquid-liquid phase separation (LLPS) to form coacervates that mimic cellular transport and regulatory mechanisms, allowing for efficient delivery of nucleic acids into cells [4][8]. - Upon entering the cell, the coacervates disassemble, releasing nucleic acids that perform their biological functions while the carrier proteins are naturally degraded [11]. Group 4: Versatility and Applications - ProteanFect is capable of efficiently transfecting various cell lines and challenging primary cells without the need for viral packaging or electroporation, making it suitable for a wide range of applications [14][16]. - The product supports multiple experimental scenarios, including overexpression, knockdown, knockout, and co-transfection, with a simplified component structure that enhances its usability [21][22]. Group 5: Performance and Efficiency - ProteanFect demonstrates high loading capacity and expression levels, allowing for the delivery of larger nucleic acid fragments and multiple biomolecules simultaneously, thus expanding the possibilities for gene therapy and complex gene regulation studies [24][26]. - The preparation process for ProteanFect is straightforward, requiring only a one-minute mixing of protein and nucleic acids before cell incubation, which significantly saves time and costs in experimental workflows [29]. Group 6: Customer Support and Value Proposition - The company provides not just high-quality research products but a complete solution that includes pre-sale customization and post-sale technical support, aiming to eliminate technical barriers for researchers [33].