山东农业大学发表最新Cell论文，破解世纪难题

Core Viewpoint - The research published in the journal Cell reveals the molecular mechanisms behind the reprogramming of single somatic cells into totipotent states during plant regeneration, addressing a century-old scientific challenge in understanding plant cell totipotency [3][5][11]. Group 1: Research Findings - The study demonstrates that LEAFY COTYLEDON2 (LEC2) can reprogram somatic epidermal cells into totipotent somatic embryonic cells [9]. - LEC2 and SPEECHLESS (SPCH) jointly activate local auxin biosynthesis through targeting TAA1 and YUC4, which is crucial for the specification of somatic embryonic cells [8][9]. - The GMC-auxin intermediate state marks the transition of guard cells from differentiation to totipotency, highlighting the role of transcriptional reprogramming and auxin signaling in this process [9][11]. Group 2: Methodology - The research utilized time-resolved live imaging, single-nucleus RNA sequencing (snRNA-seq), and laser capture microdissection combined with RNA sequencing (LCM-RNA-seq) to uncover the fate decision point in the developmental pathway [7]. - The study confirmed that the MMC (multicellular meristematic cell) is the origin of somatic embryos, and auxin biosynthesis mediated by TAA1/YUC is essential for totipotency and embryogenesis [8]. Group 3: Implications - This research not only solves the mystery of plant cell totipotency but also provides a new theoretical foundation for crop genetic improvement and efficient regeneration [5][11]. - The findings enhance the understanding of the fundamental principles of plant cell development and offer new strategies and tools for precise regulation of plant regeneration and targeted improvement of crop traits [11].