西湖大学发表最新Cell论文

Core Viewpoint - The research introduces a revolutionary toolset of time-resolved fluorescent proteins (tr-FP) that expands the application of fluorescent microscopy in both temporal and spectral domains, providing a new solution for integrating system complexity and quantitative accuracy in biological research [4][6]. Group 1: Research Findings - The study reports a series of time-resolved fluorescent proteins (tr-FP) with rationally tunable lifetimes, developed without affecting the spectral properties of the fluorescent proteins [5]. - These tr-FPs enable temporal-spectral resolved microscopy, achieving simultaneous imaging of nine different proteins in live cells and correlating various cellular activities with the cell cycle [5]. - The tr-FPs facilitate multiplexing super-resolution microscopy, allowing the visualization of four proteins simultaneously and enabling quantitative studies in cellular proteomics [5][7]. Group 2: Technical Advancements - The research demonstrates that mutations can alter the non-radiative decay of tr-FPs, which span the visible spectrum and possess a wide range of fluorescence lifetimes suitable for microscopy [7]. - The tr-FPs support advanced imaging techniques such as STED-FLIM (Stimulated Emission Depletion Fluorescence Lifetime Imaging Microscopy) and protein chemometrics for quantitative analysis [7].