Nature子刊：王冕/高绍荣/李婉露合作开发新型微孔微凝胶支架，解决干细胞递送难题

Core Viewpoint - The article discusses the development of a novel injectable micropore-forming microgel scaffold (PSMM) for the transplantation of neural progenitor cells (NPCs) to enhance recovery from ischemic stroke, highlighting its potential in improving cell survival and vascularization [3][10]. Group 1: Research Background - Stroke is a leading cause of death and disability globally, with ischemic stroke accounting for 87% of cases [2]. - Stem cell therapy is gaining attention for its potential to promote neural recovery post-stroke, but challenges remain regarding the survival and integration of NPCs after transplantation [2]. Group 2: Development of PSMM - The research team developed the PSMM scaffold using a phase separation method combined with microfluidics, resulting in microgel with controllable size and micron-sized pores [5]. - Compared to nanometer-sized pore microgels, the PSMM significantly enhances NPC density, uniformity, and activity, thereby improving cell loading capacity [5]. Group 3: Enhanced Properties of PSMM - The introduction of Matrigel/Collagen I (M/C) into the microgel scaffold improves injectability and integration with damaged tissue, while also enhancing vascularization [8]. - In vitro experiments showed that the vascular length in the PSMM group was 1.48 times greater than that in the MM group, indicating superior vascularization effects [8]. Group 4: In Vivo Results - In a rat model of ischemic stroke, the PSMM scaffold loaded with NPCs significantly improved cell survival, proliferation, and neuronal differentiation, leading to enhanced vascular regeneration and reduced glial scar formation [10]. - After 28 days, notable improvements in neurological function were observed in the treated rats [10]. Group 5: Implications and Future Directions - The PSMM scaffold represents a promising platform for stem cell encapsulation, offering high throughput, biocompatibility, and injectability, which addresses challenges in stem cell delivery for stroke treatment [11]. - This research lays a solid foundation for innovative therapies for neurodegenerative diseases [11].