82.1 g/L！山大祁庆生/侯进教授团队高效合成β-法尼烯

Core Viewpoint - β-Farnesene is an important sesquiterpene compound found in plant essential oils, used as an aphid control agent and has excellent properties as a biofuel. Its wide application significantly expands market demand [1]. Group 1: Research Findings - The research team from Shandong University published a study in the Chemical Engineering Journal, achieving a record production of β-farnesene at 82.1 g/L through global metabolic rewiring in Yarrowia lipolytica [2]. - The study introduced a non-oxidative glycolysis (NOG) pathway in Yarrowia lipolytica and blocked the core glycolysis, leading to a strain specifically for β-farnesene production, despite initial growth defects [4]. - Adaptive laboratory evolution and multi-omics analysis revealed that simultaneous inactivation of carbon repression factors Reg1 and glucose kinase Glk1 could activate the Snf1 kinase-mediated carbon signaling pathway, coordinating the NOG pathway with the TCA cycle [4][5]. Group 2: Production Efficiency - The research demonstrated the core role of Snf1 kinase in global metabolic rewiring, achieving a high yield of β-farnesene with a conversion rate of 0.21 g/g substrate, while completely eliminating citrate overflow and significantly reducing byproduct accumulation [5]. - The study highlights the potential of "energy homeostasis reconstruction" to address metabolic rigidity in microbial cell factories, providing important references for the efficient biosynthesis of terpenoid compounds [5].