Nature：华人团队开发出细胞内的“生命年轮”，利用AI设计蛋白记录并解读单个细胞历史，精度达小时级

Core Viewpoint - The article discusses the development of GEMINI, a groundbreaking intracellular recording platform that allows for precise temporal resolution of cellular activities, providing unprecedented tools for understanding physiological and pathological processes in organisms [2][4]. Group 1: GEMINI Overview - GEMINI stands for Granularly Expanding Memory for Intracellular Narrative Integration, designed to capture cellular events in a predictable manner, akin to tree rings, with a temporal precision of hours [3][6]. - The platform utilizes computationally designed protein assemblies as memory devices within cells, addressing long-standing challenges in biological research [6][8]. Group 2: Technical Features - GEMINI consists of three key components: scaffold subunits for stable growth, reporter subunits that convert cellular events into recordable fluorescent signals, and timestamp subunits that provide a temporal scale for the recorded events [8][9]. - The system's unique structure allows for isotropic growth, minimizing mechanical interference with cells and enabling accurate signal reading in all directions [9][24]. Group 3: Temporal Resolution and Applications - GEMINI achieves impressive temporal resolution, with 75.9% of single particle decoding results within ±1 hour of actual time, and the ability to distinguish events occurring as close as 15 minutes apart [14][15]. - The platform has successfully recorded physiological signals, including NF-κB signaling dynamics, and has been applied in vivo to capture neuronal activity in mouse brains without affecting their normal functions [16][20]. Group 4: Advantages and Future Prospects - Compared to previous technologies, GEMINI's modular design allows for adaptability to different signaling pathways, and its imaging readout retains spatial information, offering advantages over sequencing methods [24][25]. - The successful development of GEMINI paves the way for high-throughput cellular history analysis, marking a new era in cell biology research with potential applications in precision medicine [25].