Core Viewpoint - The Fourth Synthetic Biology and Green Bio-Manufacturing Conference (SynBioCon 2025) was held in Ningbo, focusing on advancements in synthetic biology, green manufacturing, and the integration of artificial intelligence in these fields [2][3]. Group 1: Conference Overview - The conference featured over 60 experts and corporate leaders, with more than 40 presentations across four main sessions: macro forum on bio-manufacturing, green chemistry and new materials, AI applications in bio-manufacturing, and future food & agriculture [2]. - Additional activities included a youth forum, high-level discussions on bio-manufacturing, and a closed-door seminar on the "2025 Blue Book for AI Empowerment in Bio-Manufacturing" [2]. Group 2: Keynote Highlights - Zhang Xian'en, Dean of the Shenzhen University Synthetic Biology Institute, emphasized the rapid development of synthetic biology over the past decade, highlighting the challenges posed by the complexity of biological systems and the role of AI in enhancing design efficiency [11]. - Chen Luoping, Director of the Electronic Information Research Institute, noted that AI combined with bio-manufacturing presents significant opportunities for industry advancement, particularly in enzyme design and strain construction [12][14]. Group 3: Technological Innovations - Chen Haibin, Vice President of Ningbo Enzyme Bioengineering Co., discussed the company's transition from technological innovation to product innovation, achieving industrial-scale production of small organic acids and bio-based nylon [16]. - Feng Yan, a professor at Shanghai Jiao Tong University, presented breakthroughs in enzyme molecular design and gene editing, which facilitate the creation of synthetic pathways for high-value compounds [20]. Group 4: Specialized Forums - The green chemistry and new materials forum featured eight experts discussing advancements in bio-manufacturing of polyamide materials and the development of key monomers [21][24]. - Li Zhen, co-founder of Suzhou Juweiyuan Biotechnology Co., introduced the company's innovative use of straw as a raw material for producing bio-based succinic acid at a scale exceeding 10,000 tons [30]. Group 5: Industry Collaboration - A signing ceremony established a joint laboratory between Shenzhen University and Ningbo Enzyme Bioengineering Co., aimed at fostering collaboration in synthetic biology research [4][6]. - The conference also included discussions on the establishment of innovation platforms and the cultivation of high-growth companies in the biomedicine and bio-agriculture sectors [6].