Core Insights - The article highlights significant breakthroughs in flow cytometry expected in 2025, with spectral flow cytometry emerging as the mainstream technology, driven by multi-laser, high-channel, and full-spectrum systems that enhance multi-color detection capabilities [1][2] - New technologies such as imaging flow cytometry, Raman spectral flow cytometry, and nano flow cytometry are making notable progress, indicating a shift from "phenotypic analysis" to "structural and functional integration analysis" [2] Product Releases - A total of 13 new flow cytometry products from 11 manufacturers were reported for release in 2025, showcasing advancements in various types of flow cytometry technologies [3] - Key products include: - BD's FlowJo™ v11 flow analysis software, which features a new code architecture and intuitive interface for high-dimensional data mining [10] - Cytek's Aurora™ Evo system, which enhances the capabilities of the previous Aurora system with improved sensitivity and resolution [32] - BaySpec's Endogen 3 Implex, a high-throughput flow cytometer based on coherent Raman spectroscopy, capable of measuring at a rate of up to 24,000 spectra per second [26] Technological Advancements - The FACSDi scover™ A8/A8 FX imaging spectral flow cytometer by BD allows for high-definition imaging of cell morphology and subcellular structures, enhancing the understanding of cell function [7] - The OMNIS ONE by Cytek integrates a 5-laser, 79-channel full-spectrum detection system, achieving over 99% cell viability and 95% single-cell rate post-sorting [13] - The iQue® 5 high-throughput flow cytometer from Sartorius supports up to 27 channels and is designed for continuous operation, significantly reducing operational maintenance time [29] Application Scope - The advancements in flow cytometry technologies are applicable across various fields, including basic research, clinical tumor immune microenvironment analysis, and pathogen fine typing in infectious diseases [9] - The new products are designed to meet diverse laboratory needs, from high-dimensional immune analysis to clinical diagnostics, enhancing the capabilities of researchers and clinicians alike [24][30]