Core Insights - The research team has successfully decoded the frequency modulation (FM) signal processing in bacteria, achieving a significant breakthrough in synthetic biology [2][3] - The study reveals that frequency modulation can enhance information entropy by approximately 2 bits compared to traditional amplitude modulation, allowing for precise coordination of multi-gene systems [2][5] Group 1: Research Findings - The research addresses a core challenge in synthetic biology: achieving precise coordination of multiple genes, which has traditionally relied on amplitude modulation [3] - The team utilized synthetic biology engineering techniques to reconstruct a simplified cAMP signaling pathway in Pseudomonas aeruginosa, creating a frequency decoding cAMP circuit (FDCC) [3][4] - The FDCC system consists of three functional modules: a waveform converter, a threshold filter, and an integrator, each operating within its optimal frequency range [4] Group 2: Methodology and Technology - The research team developed a multi-level theoretical framework that connects microscopic molecular reactions to macroscopic system behaviors, achieving a correlation of 99.2% between theoretical predictions and experimental data [5] - The automated experimental platform at the Shenzhen Synthetic Biology Research Major Science Facility enabled high-throughput processing of samples, demonstrating excellent stability and reproducibility over extended experiments [5][6] Group 3: Implications for Synthetic Biology - The findings indicate that frequency modulation significantly enhances bacterial information processing capabilities, transforming it from a "single-lane" to a "multi-lane" system, thus allowing for more precise control of gene expression [6] - This advancement opens new dimensions for synthetic biology, providing more accurate and complex regulatory mechanisms that can improve metabolic engineering and responsive design in cell therapy [6]