Core Viewpoint - The research presents a novel "Plug-in" breeding strategy for potato late blight resistance, addressing a significant global food security threat posed by the pathogen Phytophthora infestans [2][6]. Group 1: Research Findings - The study constructed a potato disease resistance gene resource library, revealing the differentiation characteristics of two types of resistance genes and their co-evolution with pathogens [3]. - The research team identified three new late blight resistance genes through comparative and evolutionary genomics, proposing a new breeding strategy for developing durable resistant potato varieties [3][4]. - A comprehensive "potato NLRome" was created, encompassing 39,211 NLR class resistance genes from 31 wild and 21 cultivated potato genomes, representing a wide diversity of both wild and cultivated potatoes [4]. Group 2: Genetic Insights - The team successfully cloned the Rpi-cph1 and Rpi-cjm1 genes, with the former previously only found in American black nightshade, and demonstrated the widespread presence of atypical NLR integrated domains in the NLRome [4]. - The identification of the Rpi-brk1 gene, which recognizes pathogen effector proteins through its heavy metal-associated (HMA) domain, suggests that the "Plug-in" strategy can broaden the resistance spectrum of potato NLR genes [4]. Group 3: Implications for Agriculture - The findings provide a new solution for tackling potato late blight, a global challenge, and establish a paradigm for discovering resistance genes through comparative and evolutionary genomics [6].