As filed with the Securities and Exchange Commission on October 10, 2025. Registration Statement No. 333- UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.C. 20549 FORM S-1 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 BULLFROG AI HOLDINGS, INC. (Exact name of Registrant as specified in its charter) (State or other jurisdiction of incorporation) (Commission File Number) Nevada 001-41600 84-4786155 (I.R.S. Employer Identification No.) 325 Ellington Blvd, Unit 317 Gaithersburg, MD 2087 ...

Core Insights - BullFrog AI Holdings, Inc. has entered a commercial phase in collaboration with Sygnature Discovery, unlocking a potential revenue opportunity of $15–$30 million through 2028 [1][2][3] Group 1: Commercial Launch - The sales kickoff for BullFrog Data Networks™ occurred on September 12, marking a significant milestone for the company [1] - Sygnature's global business development team has completed training to effectively market BullFrog Data Networks™ [2] Group 2: Technology and Applications - BullFrog Data Networks™ utilizes AI and machine learning to assist researchers in navigating complex datasets, with applications in target identification, mechanism-of-action elucidation, patient stratification, drug repurposing, and clinical trial optimization [3][4] - The platform aims to enhance R&D efficiency and unlock the full potential of biopharma data [4] Group 3: Company Background - BullFrog AI focuses on advancing drug discovery and development through collaborations with leading research institutions and employs causal AI alongside its proprietary bfLEAP™ platform [4] - Sygnature Discovery is a prominent contract research organization with over 1,000 employees, specializing in drug discovery across various therapeutic areas [5]

Core Insights - BullFrog AI Holdings, Inc. is leveraging AI and machine learning to enhance drug development processes, focusing on reliable automation with human oversight [1][4] Webinar Details - A live webinar titled "Clinical Data Analysis with Agents: Reliable Automation with Human Oversight" will be presented by Dr. Juan Felipe Beltrán Lacouture on October 6, 2025, at 11:00 am EDT [1][7] - The webinar aims to showcase how BullFrog AI's platforms, bfPREP™ and bfLEAP™, can transform unstructured clinical data into structured datasets for advanced analysis [2][6] Technology and Methodology - BullFrog AI has successfully converted over 10,000 pages of unstructured clinical PDFs into an OMOP-structured dataset, facilitating machine learning applications [2] - The company emphasizes a balance between automation speed and the necessary human oversight to ensure data integrity and reliability [3][4] - The approach focuses on verified automation rather than full automation, aiming to enhance clinical research workflows while maintaining quality control [3] Commitment to Data Integrity - The CEO of BullFrog AI, Vin Singh, highlighted the company's commitment to advancing data integrity and reliability in drug development, showcasing the practical applications of their technologies [4]

Core Insights - BullFrog AI's bfLEAP™ platform aims to improve drug discovery success rates in a market where nearly 90% of drug candidates fail in clinical trials [1][2][4] - The company emphasizes a biology-native AI framework that provides transparency and causality in its analytics, contrasting with traditional black-box AI models [2][3] Company Overview - BullFrog AI Holdings, Inc. is a technology-enabled drug development company utilizing AI and machine learning to enhance pharmaceutical and biologic development [1][7] - The company has developed bfLEAP™, a proprietary platform designed to handle complex biomedical data and improve therapeutic decision-making [1][2] Market Positioning - The AI in drug discovery market is projected to exceed $35 billion by 2034, positioning BullFrog AI's bfLEAP™ as a leading solution that offers scientific clarity beyond mere automation [3] - The platform is part of BullFrog's broader Data Networks™ Solutions Library, which includes tools like bfPREP™ for data preparation [3][4] Technological Differentiation - bfLEAP™ is built to address the challenges of "short and wide" datasets and biological non-linearity, providing actionable insights throughout the drug development lifecycle [2][3] - The platform employs causal AI and combinatorial modeling to manage high-dimensional data effectively, correcting misleading patterns in biological datasets [2][3] Application in Drug Development - In early discovery, bfLEAP™ helps identify targets with high mechanistic potential based on molecular data [6] - During preclinical and Phase I trials, it detects subpopulations likely to respond to treatment, and in late-stage trials, it stratifies patients by genetic and behavioral variables [6]

Core Insights - BullFrog AI Holdings, Inc. has announced a strategic collaboration with Sygnature Discovery to enhance drug development using AI and machine learning [1][2] - This partnership is expected to generate between $15 million and $30 million in revenue for BullFrog AI through 2028 [2] Company Overview - BullFrog AI is a technology-enabled drug development company that utilizes AI and machine learning to improve the development of pharmaceuticals and biologics [1] - The company’s proprietary platform, BullFrog Data Networks™, is powered by the bfLEAP™ engine and aims to provide AI-driven data insights [1][3] Collaboration Details - The collaboration with Sygnature Discovery will introduce BullFrog Data Networks™ to Sygnature's global client base, enhancing brand recognition and user uptake [2][3] - Sygnature Discovery is a UK-based contract research organization specializing in drug discovery, and this partnership will complement their existing capabilities [4] Platform Capabilities - BullFrog Data Networks™ accelerates the exploration of complex datasets, aiding in early target identification, mechanism-of-action elucidation, patient stratification, drug repurposing, and clinical trial optimization [3] - The platform is designed to meet the needs of small to mid-sized biopharma companies, which are often underserved by current bioinformatics solutions [4]

As filed with the Securities Exchange Commission on January 29, 2024 Registration No. 333-____________ UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM S-1 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 BULLFROG AI HOLDINGS, INC. (Exact Name of Registrant as specified in its charter) (State or other Jurisdiction of Incorporation or Organization) (Primary Standard Industrial Classification Code Number) Nevada 7374 84-4786155 (I.R.S. Employer Identification No.) 325 Elling ...

As filed with the Securities Exchange Commission on February 13, 2023 Registration No. 333-267951 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Amendment No. 5 to FORM S-1 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 BULLFROG AI HOLDINGS, INC. (Exact Name of Registrant as specified in its charter) (State or other Jurisdiction of Incorporation or Organization) Nevada 7374 84-4786155 323 Ellington Blvd, Unit 317 Gaithersburg, MD 20878 Tel. (240) 658-6710 (Address, includin ...

As filed with the Securities Exchange Commission on January 10, 2023 Registration No. 333-267951 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Amendment No. 4 to FORM S-1 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 BULLFROG AI HOLDINGS, INC. (Exact Name of Registrant as specified in its charter) (State or other Jurisdiction of Incorporation or Organization) Nevada 7374 84-4786155 Classification Code Number) 323 Ellington Blvd, Unit 317 Gaithersburg, MD 20878 Tel. (240) ...