Core Viewpoint - Stylus Medicine, a biotechnology company, has completed a $85 million Series A funding round to develop revolutionary in vivo gene therapies, focusing on CAR-T therapies for cancer, autoimmune diseases, and genetic disorders [2][4]. Group 1: Company Overview - Stylus Medicine was founded by Patrick Hsu and others from the Arc Institute/University of California, Berkeley, and has attracted investments from notable firms such as RA Capital, Kholsa Ventures, and Johnson & Johnson [2][4]. - The company is led by Dr. Emile Nuwaysir, who has extensive experience in cell and gene therapy, previously serving as CEO of BlueRock Therapeutics, which was acquired by Bayer for $1 billion [7]. Group 2: Technology and Innovation - Stylus Medicine's technology is based on recombinant enzyme techniques that allow for precise integration of therapeutic payloads into safe harbor sites in the human genome, avoiding double-strand breaks [4][5]. - The initial focus is on developing in vivo CAR-T therapies using lipid nanoparticles (LNP) to deliver therapeutic payloads directly to immune cells, aiming for precise and durable CAR-T cell generation [4][5]. - The company utilizes large serine recombinases (LSR) that can integrate large DNA fragments (over 7kb) into the human genome, significantly enhancing the precision of gene editing compared to traditional viral methods [12][13]. Group 3: Research Contributions - The founding team published a paper in Nature Biotechnology in October 2022, proposing a new method for large fragment DNA integration using recombinant enzymes [9]. - Recent research has expanded the diversity of known LSRs by 100 times, providing new tools for genome engineering and treatment of genetic diseases [12]. - The technology allows for targeted, predictable insertion patterns without harmful DNA double-strand breaks, making it a safer alternative for gene therapy applications [5][12].