Core Insights - The research team successfully decoded the process of leaf senescence in Arabidopsis at the single-cell level, utilizing self-developed single-cell omics and spatiotemporal omics technologies [1][2] - A comprehensive single-cell atlas was constructed, encompassing 20 tissue samples and 913,769 high-quality single-cell nuclear transcriptomes, identifying 38 cell types [1] - The study established aging and youth indices, identifying 1,856 core aging-related genes and 1,875 youth-related genes, enabling quantitative assessment of leaf senescence at single-cell resolution [2] Group 1 - The research revealed key cell types and gene dynamics during leaf senescence, laying the groundwork for future studies on leaf aging [1] - A co-expression gene regulatory network for leaf development was constructed based on aging and youth indices, highlighting several key node genes that may play significant roles in leaf senescence [2] Group 2 - The study found that nutrient transfer during leaf senescence is associated with a complex carbon-nitrogen transport system, with specific genes showing high cell-type specificity in vascular tissues [3] - Key genes responsible for transporting sugars and amino acids from leaves to other parts of the plant were identified, providing insights into the mechanisms of nutrient distribution during leaf aging [3]