Core Viewpoint - The article discusses the development of a novel spatial multi-omics technology, Spatial-DMT, which enables the simultaneous mapping of DNA methylation and gene expression at near single-cell resolution, enhancing the understanding of tissue biology and its implications in development and disease [2][5][7]. Group 1: Technology Development - The research team from the University of Pennsylvania developed Spatial-DMT, a new technique for spatial profiling of DNA methylome and transcriptome in tissues [2]. - This technology allows for the generation of high-quality DNA-RNA dual-modal tissue maps, revealing how DNA methylation interacts with transcription to define cell identity and drive developmental programs [5][6]. Group 2: Biological Implications - DNA methylation is a key epigenetic mechanism that regulates gene expression and cell states, with abnormal patterns linked to various diseases, including cancer and autoimmune disorders [4]. - The study highlights the dynamic changes in DNA methylation across different cell types and developmental stages, providing insights into the molecular definitions of cell identity during mammalian development and brain function [5][6]. Group 3: Research Applications - The Spatial-DMT technology was applied to mouse embryos and postnatal mouse brains, allowing for the reconstruction of dynamic changes in the epigenome and transcriptome during embryonic development [6]. - The integration of spatial maps from different developmental stages reveals details of transcriptional regulation mediated by sequence, cell type, and region-specific methylation [6][7].