Core Insights - The article discusses a groundbreaking research study that successfully established haploid embryonic stem cells (haESC) in cattle and sheep, overcoming previous limitations in breeding these ruminants [3][4][5] - The study introduces a novel breeding strategy combining haESC, spermatid-like modifications, and non-integrative prime editing (ePE), leading to significant advancements in genetic editing of livestock [3][9] Group 1: Research Breakthroughs - The research team successfully created haploid embryonic stem cell lines from cattle and sheep, which exhibit pluripotent characteristics and can differentiate into three germ layers [4] - The innovative "Pro-iCHI" technique, which involves injecting haESC into oocytes, significantly improved the full-term development rate of embryos [4][5] - The study achieved a 100% editing efficiency for the MSTN gene, which is known to inhibit muscle growth, leading to a potential increase in muscle mass of 20%-30% in cattle and sheep [5][6] Group 2: Implications for Livestock Breeding - Traditional methods for gene editing in livestock resulted in a low birth rate of less than 5%, but the new Pro-iCHI technique combined with ePE achieved a birth rate of 13.3% for genetically edited animals [5] - The research opens new pathways for efficient breeding of genetically modified livestock, potentially transforming the agricultural industry [9] - The findings were highlighted in a related article in Nature Biotechnology, emphasizing the potential for rapid generation of genome-edited farm animals from haploid stem cells [6]