Core Viewpoint - The article emphasizes the significance of the Cre-loxP system in gene editing research, highlighting its precision and efficiency, and announces a series of courses aimed at addressing practical issues related to the application of Cre tools in research settings [1][2]. Summary by Sections 1. Overview of Cre-loxP System and AAV - The Cre-loxP system has become a standard tool in laboratories due to its effective gene editing capabilities [1]. - The upcoming course will focus on the integration of the Cre-loxP system with AAV technology, exploring core principles and optimization strategies [2][4]. 2. Course Details - A course titled "Application of Cre-loxP System Combined with AAV" will be held on June 12, featuring discussions led by senior product managers from the company [2]. - Participants are encouraged to submit questions for targeted answers during the live session, with incentives such as a chance to win a massage device [2][6]. 3. Technical Exploration - The course will cover why some literature opts for AAV delivery of Cre enzymes over traditional methods, and the unique advantages of AAV-Cre in specific research contexts [4]. - Key considerations in designing AAV-Cre vectors, such as promoter selection and dosage optimization, will be discussed [4]. 4. Knowledge Recap - The course will include a review of foundational knowledge regarding the Cre-loxP system and its applications [7][10]. - Participants will gain insights into typical application cases and the underlying experimental design strategies that lead to high-impact publications [2][7]. 5. Instructor Background - The instructors, Liu Fanrui and Xiong Zehao, bring extensive experience in molecular biology and AAV gene therapy, respectively, ensuring a knowledgeable presentation [13][15]. 6. Additional Resources - A comprehensive manual titled "Cre-loxP Recombinase System User Manual" will be available for free, providing detailed guidance on the system's application [13].