Core Viewpoint - The article emphasizes the importance of NFAT (Nuclear Factor of Activated T-cells) reporter gene cell lines as essential tools for studying immune signaling pathways and drug screening, highlighting the challenges in developing high specificity and sensitivity systems for practical applications [4][5]. Group 1: NFAT Reporter Gene Cell Lines - NFAT reporter gene cell lines are crucial for real-time monitoring of pathway activity, reflecting the functional state of immune cells [4]. - The challenges faced in constructing effective NFAT reporter systems include insufficient signal sensitivity, background noise interference, expression heterogeneity, and limitations in application scenarios [5]. Group 2: Online Course Announcement - An online course titled "NFAT Reporter Gene Cell Lines: Decoding Immune Signals and Empowering Drug Development" is scheduled for August 14, featuring advanced scientific insights from a senior scientist at the company [6]. - The course will cover the core mechanisms of NFAT-mediated signaling pathways, innovative construction strategies for NFAT reporter gene cell lines, practical case studies from CAR-T optimization to antibody drug screening, and a Q&A session [6]. Group 3: Instructor Background - The instructor, Dr. Wu Qiang, is a senior scientist specializing in in vitro pharmacology with extensive experience in target peptide and antibody drug development, particularly in the fields of endocrine and autoimmune diseases [10].