Core Viewpoint - The article emphasizes the importance of adeno-associated virus (AAV) as a crucial tool for gene delivery in fields such as gene therapy and neuroscience, while addressing the challenges researchers face in the entire process from design to implementation [3][4]. Group 1: Course Announcement - An online course titled "AAV Experimental High-Frequency Issues Breakdown: From Basic Knowledge to Practical Application" will be held on November 20, focusing on 20 core challenges faced by researchers [4][6]. - The course will be led by experienced professionals from Saiye Biotechnology, including the AAV Gene Therapy Project Manager and an AAV Solution Design Engineer [6][8]. Group 2: Learning Outcomes - Participants will gain essential knowledge about AAV-mediated gene delivery mechanisms, selection principles for serotypes and promoters based on target tissues [6]. - The course will cover the applicability and limitations of different administration routes, as well as standardization strategies for virus titer and dosage [6]. - Attendees will learn multi-dimensional effect verification methods and troubleshooting approaches when experimental results are unsatisfactory [6]. Group 3: Company Background - Saiye Biotechnology, established in 2006, is a national high-tech enterprise and recognized as a "little giant" specializing in the life sciences sector, committed to supporting global life sciences with excellent models and services [16].