Core Viewpoint - The article discusses a groundbreaking study that developed a high-speed 3D imaging technology for mapping the peripheral nervous system (PNS) in mice, significantly enhancing the understanding of its complex structure and functions [4][10]. Group 1: Technological Advancements - The research team from the University of Science and Technology of China introduced a novel imaging technique called blockface-VISoR, achieving subcellular resolution in whole-mouse imaging within 40 hours, which is several times faster than existing methods [4][10]. - This technology allows for the visualization of nerve pathways and connections in the PNS, which has been challenging due to the complexity and size of the mammalian body [3][10]. Group 2: Research Findings - The study successfully created a detailed peripheral nerve map, revealing intricate structures such as spinal motor and sensory nerves, visceral sympathetic nerves, and their interactions with various non-neural tissues and organs [13][21]. - The research utilized multiple labeling techniques, including fluorescent, immuno, and viral markers, to visualize different types of nerves, providing unprecedented insights into the PNS [12][19]. Group 3: Implications and Future Directions - The findings from this research are expected to facilitate a better understanding of the regulatory networks of the peripheral nervous system and the mechanisms of related diseases [4][10]. - The research team plans to share the image datasets online and is working on a platform for researchers to explore these data, which may offer new insights even to professional anatomists [22].