同一位PI有两个课题，不同的科学问题，细胞模型选择分道扬镳： ⭐课题A： 验证某天然产物对「神经母细胞瘤细胞增殖与分化」的抑制作用，计划高通量筛药并锁定靶点基因。 选用 人SH-SY5Y细胞系 ，可无限扩增、表型清晰；筛到有效浓度后，立即在该细胞系上做基因敲除或过表达，验证靶点必 要性。 问题1：是否必须「人体」遗传背景？ ⭐课题B： 接着回答「该产物是否通过改善神经干细胞微环境，从而促进内源性神经再生」。 将同批药物处理后的SH-SY5Y条件上清，作用于 原代小鼠神经干细胞（mNSC） ，测神经球形成率与分化神经元比例；若 神经球增大、NeuN+细胞增多，即证实「药物→分泌组→干细胞微环境」上下游因果链。 "胞"罗万象，又不知如何选择？赛业生物发起 「细胞模型选型速配」助攻 ，扫描下面二维码提交您的细胞实验需求，我们将 根据您的实验目的、修饰方式、预期周期等维度， 从海量细胞资源中挑出"最适合的那一款"——精准匹配您的细胞解决方案 。此外，我们将挑选出80份符合要求的提交用户，送出 第十五届全运会吉祥物盲盒、百元京东卡、定制雨伞等精美好礼 。 活动细则： 1、本次活动仅针对高校、医院(非卫生院)、研究所、检 ...

Core Viewpoint - The article emphasizes the importance of NFAT (Nuclear Factor of Activated T-cells) reporter gene cell lines as essential tools for studying immune signaling pathways and drug screening, highlighting the challenges in developing high specificity and sensitivity systems for practical applications [4][5]. Group 1: NFAT Reporter Gene Cell Lines - NFAT reporter gene cell lines are crucial for real-time monitoring of pathway activity, reflecting the functional state of immune cells [4]. - The challenges faced in constructing effective NFAT reporter systems include insufficient signal sensitivity, background noise interference, expression heterogeneity, and limitations in application scenarios [5]. Group 2: Online Course Announcement - An online course titled "NFAT Reporter Gene Cell Lines: Decoding Immune Signals and Empowering Drug Development" is scheduled for August 14, featuring advanced scientific insights from a senior scientist at the company [6]. - The course will cover the core mechanisms of NFAT-mediated signaling pathways, innovative construction strategies for NFAT reporter gene cell lines, practical case studies from CAR-T optimization to antibody drug screening, and a Q&A session [6]. Group 3: Instructor Background - The instructor, Dr. Wu Qiang, is a senior scientist specializing in in vitro pharmacology with extensive experience in target peptide and antibody drug development, particularly in the fields of endocrine and autoimmune diseases [10].