据介绍，研究团队通过创新性融合基因组碱基编辑技术与液滴微流控超高通量筛选策略，构建出高效降 解木质素并同步合成油脂与蛋白的工程菌株，为木质素资源的高值化利用和绿色生物制造开辟了新路 径。 中化新网讯 近日从中国科学院天津工业生物技术研究所传来消息，该所营养资源生物合成团队实现了 以木质素为原料高效合成微生物油脂和蛋白质的技术突破，对推进秸秆等农业副产物的绿色生物制造具 有重要意义。 木质素具有复杂的三维网状结构和化学顽固性，木质素的高效生物降解与转化一直是工业生物技术领域 的一大挑战。现有技术面临降解酶系效率低、优良菌株选育周期长、细胞内碳流定向调控难等多重瓶 颈。针对这一难题，研究团队以具有木质素降解能力的棒状弯孢霉为底盘菌株，构建了铜离子诱导的 MCM5-AID碱基编辑系统，显著提升了菌株的遗传多样性。在此基础上，团队利用液滴微流控高通量 筛选技术，成功选育出漆酶活性显著提升的突变株M6。该菌株漆酶活性大幅提高，展现出优异的稳定 性和工业化应用潜力。 ...

Core Viewpoint - The research team at the Tianjin Institute of Industrial Biotechnology has made significant advancements in the bioconversion of lignin into microbial oils and proteins, addressing the challenges of lignin degradation and high-value utilization [5][7]. Summary by Sections Research Breakthroughs - The team successfully developed a genetically engineered strain, Curvularia clavata M6, which efficiently degrades lignin and simultaneously synthesizes oils and proteins. This was achieved by combining genome editing technology with high-throughput screening methods [5][6]. - The engineered strain M6 exhibited a 75% increase in laccase activity, reaching 228.58 U/L, and achieved an oil accumulation of 49% of the cell dry weight while maintaining a stable protein content of 33% [6]. Industrial Application Potential - In a 5-liter fermentation scale-up, the laccase activity further increased to 240.43 U/L, with oil content rising to 51%, indicating strong stability and potential for industrial applications [6][7]. - The research highlights the effective conversion of lignin into valuable microbial oils and proteins, providing a feasible pathway for high-value utilization of lignin resources [7][9]. Future Directions - The research is supported by national strategic technology initiatives, and the team has filed for two patents related to their findings. They will present their latest research at the upcoming NFUCon 2025 forum, focusing on biomass utilization strategies [8][9].