Core Viewpoint - The article establishes an analytical method using high-performance liquid chromatography (HPLC) to determine six phenolic substances in Conghua olives, optimizing various conditions for effective separation and detection [2][4][40]. Methodology - The method involves weighing 0.5g of dried Conghua olive powder, dissolving it in isopropyl alcohol, and subjecting it to ultrasonic extraction for 30 minutes, followed by solid-phase extraction and filtration before HPLC analysis [4][6]. - The Eclipse XDB-C18 chromatographic column was selected for separation, and a diode array detector was used to optimize the testing conditions [4][12]. Results - The study found that the peak areas of gallic acid, hydroxytyrosol, oleuropein, and elenolic acid showed good linear relationships within specified concentration ranges, with R² values greater than 0.995 [4][5]. - Detection limits for the six substances were established, with gallic acid at 2.09 mg/kg and hydroxytyrosol at 2.20 mg/kg, among others [5][33]. Recovery and Precision - The recovery rates for the substances ranged from 84% to 116%, with relative standard deviations between 0.014% and 0.15%, indicating the method's reliability [5][34]. Conclusion - The optimized method provides a reliable approach for determining the content of six phenolic substances in Conghua olives, contributing valuable insights for researchers in the field [40].

| | | 4#厂房/7#厂 | 5# 房 | 6#厂房/7#厂 | 合计产量 | | | --- | --- | --- | --- | --- | --- | --- | | | | 房扩建区域 | | 房现有区域 | | | | 1 | 原儿茶酸 | 1 | 200 | | 200 | 生物医药制造 | | 2 | 咖啡酸 | 1 | 1 20 | 1 | 150 | 生物医药制造 | | 3 | 羟基酪醇 | 1 | 30 | 1 | 30 | 食品及饲料添加剂制造 | | 4 | 烟酰胺 | 1 | 1 | 3000 | 3000 | 食品及饲料添加剂制造 | | 5 | 幣氨酸 | 1 | / | 2000 | 2000 | 食品及饲料添加剂制造 | | 6 | y-氨基丁酸 | 1 | 1 | 1000 | 1000 | 食品及饲料添加剂制造 | | 7 | 谷蓝 | 30 | 20 | 1 | 50 | 生物医药制造/食品及 | | | | | | | | 饲料添加剂制造 | | 8 | D-甘露糖 | 133.6 | 16.7 | 300 | 450.3 | 食品及饲料添加剂制造 | | 9 ...