Core Viewpoint - The article establishes an analytical method using high-performance liquid chromatography (HPLC) to determine six phenolic substances in Conghua olives, optimizing various conditions for effective separation and detection [2][4][40]. Methodology - The method involves weighing 0.5g of dried Conghua olive powder, dissolving it in isopropyl alcohol, and subjecting it to ultrasonic extraction for 30 minutes, followed by solid-phase extraction and filtration before HPLC analysis [4][6]. - The Eclipse XDB-C18 chromatographic column was selected for separation, and a diode array detector was used to optimize the testing conditions [4][12]. Results - The study found that the peak areas of gallic acid, hydroxytyrosol, oleuropein, and elenolic acid showed good linear relationships within specified concentration ranges, with R² values greater than 0.995 [4][5]. - Detection limits for the six substances were established, with gallic acid at 2.09 mg/kg and hydroxytyrosol at 2.20 mg/kg, among others [5][33]. Recovery and Precision - The recovery rates for the substances ranged from 84% to 116%, with relative standard deviations between 0.014% and 0.15%, indicating the method's reliability [5][34]. Conclusion - The optimized method provides a reliable approach for determining the content of six phenolic substances in Conghua olives, contributing valuable insights for researchers in the field [40].

Core Findings - A team from the University of California, Irvine discovered that a combination of Vitamin B3 (niacinamide) and the active component in green tea, EGCG, can rejuvenate aging brain cells and effectively clear harmful protein accumulations associated with Alzheimer's disease [1][2] - The research published in the latest issue of "Aging Science" indicates that this combination significantly enhances the levels of GTP, a key energy molecule in brain cells, and improves the ability to clear β-amyloid proteins, which are characteristic of Alzheimer's disease [1][3] Mechanism of Action - The study utilized a gene-encoded fluorescent sensor named GEVAL to monitor GTP dynamics in neurons of aged Alzheimer's model mice, revealing a continuous decline in GTP levels with age, particularly in mitochondria, which severely impacts autophagy, the process of clearing damaged components [1] - Neurons treated with niacinamide and EGCG showed GTP levels restored to those of younger cells, leading to improved energy metabolism, activation of key proteins responsible for cellular transport, and efficient clearance of β-amyloid aggregates, thereby reducing oxidative stress associated with neurodegeneration [2] Implications for Treatment - The findings suggest that the combination of these two natural dietary supplements may serve as a new tool in combating cognitive decline and Alzheimer's disease [3]