基因工程

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中国科学院团队实现染色体“精准编辑”
Ren Min Ri Bao· 2025-08-08 21:41
Core Insights - The rapid development and widespread application of genome editing technology in the life sciences provide strong technical support for basic research and application development [1] - A new programmable chromosome editing technology developed by a team from the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences allows for precise manipulation of DNA at the kilobase to megabase level in plants and animals, significantly enhancing the scale and capability of eukaryotic genome manipulation [1] Group 1 - The new technology enables multi-gene stacking editing and manipulation of genomic structural variations, opening new pathways for crop trait improvement and genetic disease treatment [1] - This technology is expected to promote the development of new breeding strategies, such as manipulating genetic linkage and regulating recombination frequency for fertility control, as well as eliminating linkage drag to fully exploit the breeding potential of superior alleles in wild germplasm [1] - Reviewers have evaluated this work as a significant breakthrough in the field of genetic engineering, with enormous application potential in breeding and gene therapy [1]
基因组编辑新利器:PCE技术实现染色体“精准编辑”
Xin Hua She· 2025-08-05 01:00
Core Viewpoint - The research team from the Chinese Academy of Sciences has developed a novel programmable chromosome-level large segment DNA manipulation technology, PCE, which enables precise editing of DNA at the kilobase to megabase level in eukaryotic genomes, representing a significant breakthrough in the field of genetic engineering [1][2]. Group 1: Technology Development - The new method addresses the core challenge of precise manipulation of large segments of DNA, which existing tools struggle with in terms of efficiency, scale, precision, and diversity [2]. - The research team has created two programmable chromosome editing systems, PCE and RePCE, which allow for precise and seamless manipulation of ultra-large segments of DNA [2][3]. - Three key technological innovations have been achieved to enhance the capabilities of large segment DNA editing, including the development of a high-throughput recombination site modification platform, optimization of Cre recombinase using artificial intelligence, and the creation of a "no trace editing strategy" called Re-pegRNA [3]. Group 2: Applications and Implications - The new technology has the potential to revolutionize crop trait improvement and genetic disease treatment by enabling the manipulation of genomic structural variations [4][6]. - In traditional breeding, desirable traits are often linked with undesirable genes, but the breakthrough in large segment DNA editing could lead to new breeding strategies that eliminate these undesirable traits while enhancing beneficial ones [4]. - The technology has already been successfully applied to create a rice variety with a precise inversion of 315 kilobases that is resistant to herbicides [5]. - In the field of genetic disease treatment, this technology may provide new therapeutic approaches for diseases caused by chromosomal abnormalities and accelerate the construction of artificial chromosomes, which is crucial for synthetic biology and other emerging fields [6].
【新华社】基因组编辑新利器:PCE技术实现染色体“精准编辑”
Xin Hua She· 2025-08-05 00:40
Core Viewpoint - The research team from the Chinese Academy of Sciences has developed a novel programmable chromosome-level large segment DNA manipulation technology called PCE, which enables precise editing of DNA from thousands to millions of base pairs, representing a significant breakthrough in the field of genetic engineering [2][3]. Group 1: Technological Breakthroughs - The PCE technology addresses the core challenge of precise manipulation of large segment DNA, which has been a limitation of existing tools in terms of efficiency, scale, precision, and diversity [3]. - The research team has created two programmable chromosome editing systems, PCE and RePCE, which allow for precise and seamless manipulation of ultra-large segment DNA [3][5]. - Innovations include a high-throughput recombination site rapid modification platform and an AI-based optimization of Cre recombinase, which enhances editing efficiency by 3.5 times [5]. Group 2: Applications and Implications - The new technology has the potential to revolutionize crop trait improvement and genetic disease treatment by enabling precise genomic structural variations [6]. - In traditional breeding, desirable traits are often linked with undesirable genes; the new method can help decouple these traits, enhancing breeding strategies [6]. - The technology has already been successfully applied to create herbicide-resistant rice with a precise inversion of 315 kilobases [7].
科学与健康|基因组编辑新利器:PCE技术实现染色体“精准编辑”
Xin Hua She· 2025-08-04 15:30
Core Insights - The article discusses a revolutionary breakthrough in genome editing technology, specifically the development of a new programmable chromosome-level DNA manipulation technique called PCE, which enables precise editing of DNA segments ranging from thousands to millions of base pairs [1][2]. Group 1: Technology Development - The research team has developed two programmable chromosome editing systems, PCE and RePCE, which allow for precise manipulation of ultra-large DNA segments, effectively addressing the "scale dilemma" in genome editing [2]. - The team has achieved three key technological innovations to enhance the capabilities of ultra-large DNA precise editing [2][3]. Group 2: Technical Innovations - The first innovation involves changing the "double-gate" system to a "single-channel" system to overcome the reversibility issue of recombination reactions caused by the inherent symmetry of Lox sites, creating a new type of Lox variant that facilitates targeted editing [2]. - The second innovation utilizes artificial intelligence to optimize the Cre recombinase, resulting in a 3.5-fold increase in recombination efficiency, thereby enhancing the overall effectiveness of the editing process [3]. - The third innovation is the development of the Re-pegRNA strategy, which acts like a smart eraser to precisely identify and eliminate residual sites that could interfere with the accuracy of genome editing [3]. Group 3: Applications and Implications - The new ultra-large DNA precise editing method is expected to open new pathways for crop trait improvement and genetic disease treatment by manipulating genomic structural variations [4]. - In traditional breeding, desirable traits are often linked with undesirable genes, but the breakthrough in large segment DNA editing could lead to new breeding strategies that control fertility and eliminate linked burdens, maximizing the breeding potential of wild germplasm resources [4]. - The research team has successfully created a rice variety with a precise inversion of 315 kilobases that is resistant to herbicides, showcasing the practical application of this technology [4].
Cell:高彩霞团队开发超大片段DNA编辑新方法,实现千碱基到兆碱基级的高效、精准、无痕编辑
生物世界· 2025-08-04 14:33
Core Viewpoint - The article discusses the revolutionary advancements in genome editing technology, particularly focusing on the development of a new programmable chromosome engineering (PCE) system that enables precise manipulation of large DNA segments ranging from kilobases to megabases, which addresses significant challenges in the field of genetic engineering [4][11]. Group 1: Technology Overview - Genome editing technology, exemplified by CRISPR and its derivatives, has made significant strides in precise editing of specific bases and short DNA fragments, but large-scale DNA editing remains a core challenge [3]. - The PCE system developed by the research team allows for efficient and precise manipulation of DNA at the chromosome level, significantly enhancing the capability to edit eukaryotic genomes [4][11]. Group 2: Technical Innovations - The research team addressed three key limitations of the Cre-Lox system, including the inherent symmetry of Lox sites, the difficulty in engineering Cre enzyme activity, and the precision of editing due to residual Lox sites after recombination [6][8]. - Innovations include a high-throughput recombination site modification platform, the development of new Lox variants with reduced recombination activity, and an engineered Cre protein variant that improves recombination efficiency by 3.5 times [6][7]. Group 3: Applications and Implications - The PCE system has successfully achieved various large-scale DNA manipulations, including the integration of an 18.8 kb DNA segment, directional replacement of a 5 kb sequence, and chromosomal inversions and deletions up to 12 Mb [7]. - This technology not only facilitates multi-gene stacking but also opens new pathways for crop trait improvement and genetic disease treatment, potentially transforming breeding strategies and synthetic biology applications [11].
中源协和: 中源协和细胞基因工程股份有限公司十一届十九次临时董事会会议决议公告
Zheng Quan Zhi Xing· 2025-07-09 08:09
Group 1 - The company held its 19th temporary board meeting on July 9, 2025, via telecommunication, with all 9 attending directors agreeing to waive the notice period due to urgency [1] - The board approved the appointment of Mr. Chen Yiqing as the secretary of the board, with a term lasting until the current board's term ends [1][2] - Mr. Chen Yiqing possesses the necessary qualifications and experience to fulfill the role of board secretary, having obtained the required training certification and meeting legal requirements [2] Group 2 - The company’s subsidiary, Beijing Zhongshan Jinqiao Biotechnology Co., Ltd., plans to donate a total of 3 million yuan, with 2 million yuan to the Peking Union Medical Foundation and 1 million yuan to the Beijing Red Cross Foundation, aimed at supporting public welfare projects [2] - This donation is part of the company's commitment to social responsibility and is expected to enhance its image and social influence [2] - The funds for the donation will come from the company's own resources and will not significantly impact its operational performance or harm the interests of shareholders, particularly minority shareholders [2]