关键 词 | SynBio团队 | 张荣珍教授）胆红素 【SynBioCon】 获 悉，近日， 江南大学生物技术学院 张荣珍教授团队 联合中国科学院上海药物研究所、美 国约翰斯·霍普金斯大学等机构 ，在《自然·通讯》（Nature Communications）上发表题为《Systemic engineering and global regulation enabling high-level bilirubin biosynthesis》的研究论文， 成功构 建了一个高效体外酶催化级联系统，将胆红素产量提升至1.7克/升，产率达95.8%，实现了该高价值化合物克 级规模的生物合成，为工业化生产奠定了坚实基础 。 研究首先厘清了从血红素到胆红素的经典两酶催化路径，并通过对血红素加氧酶、其氧化还原伴侣蛋白以及胆 绿素还原酶的系统进化分析和筛选，从众多物种中鉴定出了最高效的酶组合。实验表明，来自大鼠的HO-1与 其专一的细胞色素P450氧化还原伴侣CPR的组合，在催化血红素转化为胆绿素这一步中效率最高；同样来自 大鼠的BVR-A亚型则在后续将胆绿素还原为胆红素的步骤中表现最佳。 为进一步提升酶的可溶性表达，研究 ...

Core Viewpoint - The article discusses the revival of electrochemistry in synthetic chemistry, highlighting its potential in enzyme applications and the challenges faced in utilizing electrochemistry to unlock new catalytic modes for enzymes [2]. Group 1: Research Findings - A research team from Nanjing University published a paper in Nature on May 28, 2025, demonstrating a new catalytic function of ThDP-dependent enzymes through the integration of electrocatalysis and enzyme catalysis, achieving dynamic kinetic oxidation of aldehydes to chiral carboxylic acids [2]. - The study reported a robust electroenzymatic method for the efficient synthesis of ten (S)-arylpropionic acid anti-inflammatory drugs, such as ibuprofen and naproxen, with enantiomeric selectivity reaching 99% ee and enzyme loading as low as 0.05 mol% [4]. - Mechanistic studies revealed that the electroenzyme plays multiple roles in precise substrate recognition, accelerating racemization, and facilitating electron transfer events for kinetic matching [5]. Group 2: Recent Publications - The research team has published three papers in Nature over the past two years, including a study on December 18, 2023, that developed a dual catalytic system combining ThDP-dependent enzymes and photocatalysis, significantly expanding enzyme catalytic functions and providing new strategies for chiral precision control in chemical fields [7]. - Another publication on November 22, 2024, introduced a synergistic photobiocatalysis system for enantioselective triple radical sorting, overcoming the limitations of natural enzymes that typically catalyze only 1-2 substrates, thus empowering green biomanufacturing [10].