Core Viewpoint - The article emphasizes the integration of AI and multi-omics analysis, highlighting the importance of machine learning in biological data analysis and the educational offerings to enhance skills in this area [1][2][3]. Group 1: Course Features - The course is designed for beginners, providing a comprehensive introduction to R programming for bioinformatics analysis [1]. - It covers various popular directions in multi-omics research, including metabolomics, proteomics, microbiomics, and transcriptomics, keeping pace with scientific advancements [1]. - The teaching model includes one-on-one guidance and a flexible learning pace with live classes and recorded sessions [3]. Group 2: Course Content Overview - The first session focuses on interpreting CNS papers using Deepseek for efficient reading and summarizing multi-omics data analysis methods [2]. - Subsequent sessions cover the design of multi-omics research projects, programming basics in R, and machine learning applications in metabolomics, proteomics, and microbiomics [2][4][6]. - Advanced topics include the use of various machine learning models like xgboost, lasso, and random forests for intelligent data analysis [3][10]. Group 3: Practical Applications - The course includes hands-on experience with real CNS article source codes, allowing participants to replicate high-level research [3]. - It emphasizes the application of machine learning techniques in analyzing metabolomics and proteomics data, including regression models and network analysis [4][9]. - The integration of multi-omics data for comprehensive analysis is highlighted, showcasing the potential for significant insights in biomedical research [12][14].

Core Viewpoint - The article discusses the pervasive presence of microplastics and nanoplastics (MNP) in the environment and their potential accumulation in the human body, leading to various health risks [1][2]. Group 1: Microplastics and Nanoplastics Overview - Microplastics and nanoplastics are found in everyday items such as air, bottled water, food packaging, and takeaway containers, leading to unavoidable exposure and ingestion [2]. - Previous studies indicate that microplastics can disrupt gut microbiota balance and are associated with intestinal barrier dysfunction, inflammation, and immune imbalance [2]. Group 2: Recent Research Findings - A study published on June 10, 2025, in Nature Communications explored the interaction between polystyrene nanoplastics and the intestinal microenvironment [3][4]. - The research team utilized RNA sequencing, transcriptome analysis, and microbiome sequencing to analyze the effects of polystyrene nanoplastics on the gut environment in mice [6]. Group 3: Mechanisms of Impact - The study found that the accumulation of nanoplastics in the mouse gut altered the expression of two microRNAs (miR-501-3p and miR-700-5p), which in turn affected the expression of proteins related to gut barrier integrity (ZO-1 and MUC-13), increasing gut permeability [7]. - Nanoplastics elevated levels of specific microRNAs in extracellular vesicles from goblet cells, disrupting the expression of ZO-1 and inducing dysbiosis in the gut microbiota, particularly increasing the abundance of Ruminococcaceae associated with gastrointestinal dysfunction [8]. Group 4: Implications and Future Research - These findings reveal a mechanism by which nanoplastics can compromise gut integrity and indirectly alter gut microbiota composition, potentially leading to adverse health outcomes, although further research is needed to understand the implications for human health [10].

Core Viewpoint - The article emphasizes the significance of the Cre-loxP system in gene editing research, highlighting its precision and efficiency, and announces a series of courses aimed at addressing practical issues related to the application of Cre tools in research settings [1][2]. Summary by Sections 1. Overview of Cre-loxP System and AAV - The Cre-loxP system has become a standard tool in laboratories due to its effective gene editing capabilities [1]. - The upcoming course will focus on the integration of the Cre-loxP system with AAV technology, exploring core principles and optimization strategies [2][4]. 2. Course Details - A course titled "Application of Cre-loxP System Combined with AAV" will be held on June 12, featuring discussions led by senior product managers from the company [2]. - Participants are encouraged to submit questions for targeted answers during the live session, with incentives such as a chance to win a massage device [2][6]. 3. Technical Exploration - The course will cover why some literature opts for AAV delivery of Cre enzymes over traditional methods, and the unique advantages of AAV-Cre in specific research contexts [4]. - Key considerations in designing AAV-Cre vectors, such as promoter selection and dosage optimization, will be discussed [4]. 4. Knowledge Recap - The course will include a review of foundational knowledge regarding the Cre-loxP system and its applications [7][10]. - Participants will gain insights into typical application cases and the underlying experimental design strategies that lead to high-impact publications [2][7]. 5. Instructor Background - The instructors, Liu Fanrui and Xiong Zehao, bring extensive experience in molecular biology and AAV gene therapy, respectively, ensuring a knowledgeable presentation [13][15]. 6. Additional Resources - A comprehensive manual titled "Cre-loxP Recombinase System User Manual" will be available for free, providing detailed guidance on the system's application [13].

撰文丨王聪 编辑丨王多鱼 排版丨水成文 晚发性阿尔茨海默病 （Late-Onset Alzheimer's Disease, LOAD） 是指 65 岁之后发病的阿尔茨海默病 （AD） ，占所有阿尔茨海默病的 90% 以上。全基因组关联研究 （GWAS） 已确定了多个 与 LOAD 发病 风险增加相关的基因多态性，许多与 LOAD 风险相关的等位基因通过影响 小胶质细胞 （microglia，MG） 的先天免疫反应和脂质代谢来改变疾病的发生机制。 2025 年 6 月 9 日，西达赛奈医疗中心的研究人员在 Nature 子刊 Nature Aging 上发表了题为： Boosting angiotensin-converting enzyme (ACE) in microglia protects against Alzheimer's disease in 5xFAD mice 的研究论文。 该研究显示，在小胶质细胞中增强 ACE 表达，能够保护阿尔茨海默病小鼠模型的大脑，表明了 表达 ACE 的小胶质细胞可能作为一种治疗阿尔茨海默病的新型细胞疗法。 血管紧张素转换酶 （ACE） 是一种在 全基因组关联研究 ...

编辑丨王多鱼 排版丨水成文 生物医学研究是增进人类对健康和疾病的理解、推动药物研发以及提升临床护理水平的基础。 然而，在生物医学实验室中，科研人员往往被复杂的实验方案、庞大的数据库、五花八门的分析工具以及不停更新的海量文献所淹没。生物医学研究日益受到这 些重复且分散的工作流程的制约，让科研人员疲于奔命， 严重减缓了科学发现的速度，限制了科学创新。这凸显了科学界对根本性新方法的迫切需求——一种能 够 有效扩展科学专业知识、简化研究工作流程，并充分释放生物医学研究潜力的全新路径。 2025 年 6 月 2 日， 斯坦福大学 黄柯鑫 、 Serena Zhang 、 王瀚宸 、 屈元昊 、 陆荧洲 等研究人员领衔的团队，联合 Genentech、Arc Institute、 加州大学 旧金山分校及 普林斯顿大学等 多个顶尖研究机构，发布了一款 通用生物医学 AI 智能体 —— Biomni ，该智能体能够自主完成横跨遗传学、基因组学、微生物 学、药理学和临床医学等多个生物医学分支领域的复杂研究任务 。 Biomni 的诞生标志着 AI 在生物医学研究中从"工具使用者"向"自主决策者"的跃迁 。通过将分散的科研资源整 ...

2025 年 6 月 2 日， 斯坦福大学 黄柯鑫 、 Serena Zhang 、 王瀚宸 、 屈元昊 、 陆荧洲 等研究人员领衔的团队，联合 Genentech、Arc Institute、 加州大学 旧金山分校及 普林斯顿大学等 多个顶尖研究机构，发布了一款 通用生物医学 AI 智能体 —— Biomni ，该智能体能够自主完成横跨遗传学、基因组学、微生物 学、药理学和临床医学等多个生物医学分支领域的复杂研究任务 。 Biomni 的诞生标志着 AI 在生物医学研究中从"工具使用者"向"自主决策者"的跃迁 。通过将分散的科研资源整合为可操作的 智能体行为单元 ，不仅破解了传统 研究流程的碎片化瓶颈，更可能催生跨学科、高通量的自主 化科学发现引擎 。 该研究以： Biomni: A General-Purpose Biomedical AI Agent 为题，发表在了预印本平台 bioRxiv 上，研究团队在 biomni.stanford.edu 开放免费注册和使用 编辑丨王多鱼 排版丨水成文 生物医学研究是增进人类对健康和疾病的理解、推动药物研发以及提升临床护理水平的基础。 然而，在生物医学实验室中， ...

Core Viewpoint - The research conducted by Wuhan University focuses on the mechanism by which PCSK9 promotes the degradation of LDLR, offering new hope for the treatment of hypercholesterolemia [2][3]. Group 1: Research Findings - The study reveals that PCSK9 obstructs the recycling of LDLR, leading to its degradation in lysosomes, which is critical for understanding cholesterol metabolism [2]. - Various cell lines, including HuH7 and LDLR knockout HuH7 cells, were utilized to explore the intricate regulatory mechanisms between PCSK9 and LDLR [2]. - The research involved multiple experimental stages, including validating the impact of SNX17 on LDLR recycling and investigating how PCSK9 interferes with this process [2]. Group 2: Product Usage - The research utilized ExCell Bio FSP500, a premium fetal bovine serum, which is essential for maintaining cell growth and functionality in vitro [3]. - The choice of fetal bovine serum is crucial, as improper selection can significantly affect experimental outcomes and publication potential [6]. Group 3: Industry Standards and Safety - The International Serum Industry Association (ISIA) emphasizes the importance of traceability in serum sourcing to ensure product quality and safety [6]. - The prevalence of smuggled serum from countries with frequent bovine diseases poses significant safety risks for researchers [8]. - Recent incidents, such as the smuggling case involving 12 million yuan worth of bovine serum, highlight the need for stringent regulations and compliance in the serum industry [10]. Group 4: Company Credentials - ExCell Bio is recognized as a leading global manufacturer of fetal bovine serum, with a strong commitment to safeguarding biological safety [12][14]. - The company ensures that all serum products are traceable to their source and meet rigorous quality standards through proper documentation [14][15].

Core Viewpoint - The article discusses the development of a new thrombectomy technique called Milli-spinner thrombectomy, which significantly improves the efficiency of thrombus removal compared to existing methods, potentially transforming treatment for ischemic stroke, heart attacks, pulmonary embolism, and other thrombus-related diseases [4][5][25]. Group 1: Current Thrombectomy Techniques - Mechanical thrombectomy currently fails to clear thrombus in 10%-30% of patients, particularly those with large, fibrin-rich clots [3]. - Existing methods may cause thrombus fragmentation, leading to distal embolization and adverse outcomes [3][8]. Group 2: Milli-spinner Thrombectomy - The Milli-spinner thrombectomy technique is reported to be over twice as effective as current technologies, achieving a 90% success rate in clearing difficult thrombi on the first attempt, compared to approximately 11% with existing devices [5][7]. - This technique utilizes a unique design that applies compressive and shear forces to reduce thrombus volume without causing fragmentation [9][13]. Group 3: Mechanism and Design - The device consists of a hollow, rapidly rotating catheter with fins and slits that create a local negative pressure zone around the thrombus, allowing for effective thrombus reduction [10][15]. - The innovative design allows the thrombus to be reduced to less than 5% of its original volume, enabling red blood cells to flow freely again [16][17]. Group 4: Future Applications and Development - The research team is exploring other potential applications of the Milli-spinner device in the biomedical field, including capturing and removing kidney stone fragments [23]. - A new company has been established to expedite the development and clinical trials of this technology, aiming for rapid approval for patient treatment [24].

Core Viewpoint - The research highlights the promising efficacy and safety of anti-CD38 monoclonal antibodies in treating immune thrombocytopenia (ITP), particularly in pediatric patients, indicating a potential new treatment option in this area [1][3][11]. Group 1: Clinical Research Findings - A phase 1/2 clinical study published in NEJM demonstrated that the domestic anti-CD38 monoclonal antibody (CM313) showed good safety and durable efficacy in treating ITP, with 95% of patients achieving a platelet count of at least 50×10^9/L during treatment [1]. - In a subsequent phase 2 study, 90% of pediatric patients with relapsed or refractory ITP responded positively to Daratumumab, with a median time to platelet response of 1 week [7][11]. - The study indicated that 50% of patients maintained a durable response at 24 weeks, comparable to existing second-line therapies [8][11]. Group 2: Safety Profile - The use of Daratumumab was found to be safe, with a low incidence of serious adverse events and no treatment interruptions due to side effects [10]. - The most common adverse events included infusion-related reactions (35%) and upper respiratory infections (30%), which were manageable and did not lead to long-term complications [10].

编译丨王聪 编辑丨王多鱼 排版丨水成文 总的来说，该研究证实了 NINJ1 是膜生物力学特性的真正决定因素， 还表明了 在不同机械微环境的组织中，质膜破裂受到 NINJ1 表达差异和外部机械力的精细 调控。 论文链接 ： 质膜的完整性对于细胞功能的几乎所有方面都至关重要。 机械力 可导致质膜受损，但尚不清楚是否存在大分子在机械应变下调控质膜的完整性。 2025 年 6 月 9 日，中山大学第一附属医 院 许杰 、 向芙莉 、新泽西州立大学 Zheng Shi 及北京生命科学研究院 邵峰 院士等，在国际顶尖学术期刊 Nature 发 表了题为 ： NINJ1 regulates plasma membrane fragility under mechanical strain 的研究论文。 在这项最新研究中，研究团队构建了一个 384 孔细胞拉伸系统，能够对培养细胞施加精确且可重复的拉伸力。利用该系统，研究团队使用 10843 种 siRNA 以靶 向筛选 2726 种多重跨膜蛋白，以检测拉伸诱导的膜通透性变化。 该筛选结果将 NINJ1 蛋白质列为首要发现，该蛋白质最近被发现可调控细胞焦亡及其他溶解性细胞死亡 ...