Core Viewpoint - The article discusses the advancements in third-generation biorefining technology that utilizes one-carbon (C1) resources, such as CO2, to produce biofuels and chemicals, contributing to carbon capture and utilization, and supporting carbon neutrality goals [1][3][9]. Summary by Sections 1. Development of Biorefining Technology - The oil crisis in the 1970s spurred research and industrialization of biofuels, leading to the introduction of biorefining concepts in the 1980s [7]. - The first-generation biorefining technology, using food crops, faced challenges related to resource efficiency and competition with food production [8]. - The second-generation technology, based on lignocellulosic biomass, has potential but is hindered by high costs and technical barriers [8]. 2. Third-Generation Biorefining Technology - Third-generation biorefining aims to convert CO2 and renewable energy sources into fuels and chemicals, overcoming limitations of previous technologies [9]. - This technology has shown significant progress, with over 10 carbon fixation pathways validated, and some CO2 fixation technologies have reached commercialization [4][9]. - Examples include projects that convert industrial emissions into bioethanol, significantly reducing CO2 emissions [4]. 3. Carbon Fixation Pathways - More than 10 carbon fixation pathways have been identified, including natural pathways like the Calvin cycle and engineered pathways [11][36]. - The article details various pathways, such as the Wood-Ljungdahl pathway and reductive TCA cycle, highlighting their unique characteristics and potential for industrial application [16][33]. 4. Engineering of Carbon Fixation - Key factors influencing carbon fixation efficiency include energy sources, substrate types, and enzyme characteristics [36]. - Engineering efforts focus on optimizing microbial strains for better CO2 utilization and product yield, with examples of successful modifications in various microorganisms [38]. 5. Commercialization and Future Prospects - The commercialization of third-generation biorefining technologies is underway, with successful projects demonstrating the feasibility of using CO2 as a raw material [4][9]. - Future developments are expected to enhance the efficiency and cost-effectiveness of these technologies, contributing to sustainable bio-manufacturing [9][36].

Core Viewpoint - The article discusses the "Action Plan for Promoting High-Quality Development of the Biological Manufacturing Industry in Hainan Province (2025-2027)", which aims to enhance the biological manufacturing sector in Hainan through a structured development path focusing on marine biological manufacturing and breeding [1][2]. Group 1: Development Strategy - The "5+8+N" development path emphasizes two main areas: marine biological manufacturing and biological breeding, targeting five key sectors: biomedicine, biological food, biological agriculture, biological materials, and biological chemicals and energy [2][3]. - The plan includes establishing an innovation center for marine biological manufacturing by the end of this year and aims to cultivate several flagship products and key industrial chain projects [2][3]. Group 2: Advantages of Hainan - Hainan possesses unique advantages for developing the marine biological manufacturing industry, including rich marine resources, favorable policies from the free trade port, established platforms for deep-sea biological development, and a solid industrial foundation with a nearly 800 billion yuan output in high-end food processing and biomedicine [3][4]. - The province plans to develop marine bio-based materials using resources like blue and red algae to produce materials such as polylactic acid and carrageenan fibers [3]. Group 3: Future Goals - By the end of 2027, Hainan aims to create a "dual-core, dual-wing, multi-point" industrial spatial layout centered around Haikou and Sanya, with the goal of achieving over 100 billion yuan in biological manufacturing output and enhancing the overall quality of the industry [4][5].

Core Viewpoint - Dapu Biotech aims to create a new generation of intelligent life science solutions, focusing on the deep integration of AI and biotechnology [1] Company Overview - Dapu Biotech was founded in 2018, incubated at the Hong Kong University of Science and Technology, and is a leading enterprise in high-throughput droplet microfluidics technology in China [5] - The company has R&D and production centers in Shenzhen and Jiaxing, with a team of nearly 100 people dedicated to applying droplet microfluidics technology in biomedicine and precision medicine [5] - Dapu Biotech aims to provide a complete solution integrating the R&D and production of microfluidic chips, instruments, and reagents [5] Product Offerings - The company has commercialized multiple scientific instruments based on droplet microfluidics technology, including: - CytoSpark® Droplet Microfluidic High-throughput Screening System - OMNIdrop® System - Galaxy Single Cell Analysis System - Nebula dPCR System - Exostar Extracellular Vesicles Analysis System - These products are applicable in various fields such as antibody screening, enzyme directed evolution, strain screening, small molecule compound screening, cancer research, tumor early screening, virus quantification, and biopharmaceutical quality control [6] System Advantages - OMNIdrop® System features: - Multifunctional integration of droplet generation, fusion, reading, and sorting - Dual-drive pressure system for easy switching - High-speed droplet generation of 100 to 8000 droplets per second, with a million droplets prepared in just 10 minutes - High compatibility with commonly used biological experimental consumables [9] - CytoSpark® CSP System advantages include: - Efficient screening of complex transmembrane proteins - Unique non-activation screening technology - Breakthrough in single-experiment coverage of over one million B cells, improving efficiency by 10 to 100 times [14] Industry Events - The SynBioCon 2025 conference will be held from August 20-22 in Ningbo, Zhejiang, focusing on the integration of AI and biological manufacturing, and exploring trends in the biomanufacturing industry [18]

Core Viewpoint - The approval of D-allohexose as a new food ingredient marks a significant milestone for the domestic market, with Fuyang Bio becoming the first company to obtain production licenses, indicating a potential surge in demand for low-calorie sweeteners in various food applications [1][2][10]. Group 1: D-allohexose Overview - D-allohexose is a six-carbon ketose sugar found naturally in foods like figs and kiwis, with an energy coefficient of approximately 1.67 kJ/g [4]. - The production methods include microbial fermentation using E. coli AS10 and enzyme conversion from fructose, resulting in a product with a minimum content of 98 g/100 g [4][10]. - Recommended daily intake is ≤ 20 grams, with caution advised for infants, pregnant, and breastfeeding women due to insufficient safety data [5]. Group 2: Market Potential and Applications - D-allohexose has a sweetness level close to 70% of sucrose but only 10% of its calories, making it suitable for large-scale use in international food, beverage, and seasoning markets [6]. - The FDA and other international bodies have recognized D-allohexose as a safe substance, allowing it to be excluded from total sugars on nutrition labels, with a caloric value of 0.4 kcal/g [8]. Group 3: Fuyang Bio's Development - Fuyang Bio has established a strong research foundation for D-allohexose, collaborating with renowned experts and institutions to enhance production techniques and applications [7]. - The company has received multiple national invention patents in the field, showcasing its competitive edge in D-allohexose technology [7]. - The recent approval from the National Health Commission allows Fuyang Bio to fully enter the domestic market, indicating a strategic advantage in the burgeoning low-calorie sweetener sector [10].

Core Viewpoint - The article highlights the upcoming "Synthetic Biology and Green Bio-Manufacturing Conference 2025" (SynBioCon 2025) taking place in Ningbo, Zhejiang from August 20-22, focusing on innovative achievements in synthetic biology and bio-manufacturing [2][14]. Group 1: Event Details - The "Youth Forum on Bio-Manufacturing" will be held as a special session during SynBioCon 2025, aimed at showcasing innovative research teams, technologies, and products in the field [2]. - The forum will provide a platform for discussing scientific issues, solutions, achievements, scalability, and future directions in the research area [3]. - Registration is open for universities and research institutions to share their findings and innovations [4]. Group 2: Participation and Registration - Participants can choose to register for either the Youth Forum or the Technology Achievement Exhibition [5]. - The forum will take place on August 20, with limited seating available (only 30 seats) [6]. - The Technology Achievement Exhibition will feature 100 innovative projects in synthetic biology and bio-manufacturing, allowing for on-site display and networking opportunities [6]. Group 3: Organizing Institutions - The event is organized by Ningbo Detaizhong Research Information Technology Co., Ltd. (DT New Materials) and supported by various institutions including the Chinese Society of Biotechnology and local industry alliances [12][14].

Group 1 - The article introduces the "Green Bio-Manufacturing" National Key Laboratory, established by Beijing University of Chemical Technology, Tsinghua University, and Dabeinong Group, focusing on producing liquid fuels and important chemicals from non-food biomass resources to support the reduction of fossil resources and CO2 emissions [1][3]. - The laboratory is led by Professor Tan Tianwei, an academician of the Chinese Academy of Engineering, who has a distinguished background in education and research, having trained over 100 graduate students [1][5]. - The research team includes a diverse group of experts, such as 1 academician, 6 distinguished young scholars, and 30 professors, with established verification bases and industrial connections [5]. Group 2 - The laboratory is recruiting several postdoctoral researchers to work on synthetic biology, enzyme catalysis, fermentation engineering, and chemical simulation, with responsibilities including laboratory management [6]. - Candidates should have a PhD in relevant fields, with a maximum of 3 years since obtaining their degree, and must demonstrate independent research capabilities and teamwork spirit [6][7]. - The compensation for postdoctoral positions follows the university's standards, with salaries ranging from 180,000 to 250,000 CNY per year, and additional benefits such as housing support for certain scholars [6]. Group 3 - The Syn BioCon 2025 conference will be held from August 20-22 in Ningbo, Zhejiang, focusing on opportunities in bio-manufacturing for the bio-chemical and new materials industries [9]. - The conference will cover five key areas: AI + bio-manufacturing, green chemistry and new materials, future food, future agriculture, and beauty raw materials, aiming to explore trends and innovations in the bio-manufacturing industry [9][12]. - Participants from various sectors, including industry leaders, experts, and government representatives, are invited to discuss the future of bio-manufacturing and promote the scaling and transfer of technological achievements [9].

Core Insights - The article discusses the enhancement of acid tolerance in industrial E. coli strains, which can reduce fermentation costs by improving robustness in low pH environments [1][8]. Group 1: Research Findings - A study from South China University of Technology developed acid-tolerance modules using a "toehold switch" approach, allowing E. coli to maintain lysine production at pH 5.5 comparable to pH 6.8 [1][5]. - The acid response module consists of two plasmids: one with acid-responsive promoters expressing trigger RNAs, and another with toehold switches expressing acid tolerance-related genes [3][5]. Group 2: Mechanisms and Components - The study identified four functional groups of acid tolerance genes: proton consumption system, protein protection system, cell membrane modification and redox homeostasis system, and peroxide clearance system [3][5]. - Key genes include gadB, gadE, and others for proton consumption, and degP and sthA for protein protection and hydrogenase activity, respectively [6][8]. Group 3: Experimental Results - The research utilized a high-throughput platform to create a library of acid tolerance modules, resulting in 1140 colonies after selection, with 26 combinations showing over 120% growth compared to wild-type strains [5][6]. - Two specific combinations were introduced into an industrial lysine-producing E. coli strain, leading to increased lysine yield and glucose conversion rates [5][6]. Group 4: Implications for Industry - The strategies developed in this research provide valuable insights for enhancing the robustness and productivity of industrial strains under moderate acidic conditions, potentially benefiting the biomanufacturing sector [8].

Core Viewpoint - Synthetic biology aims to "synthesize and design life," with significant advancements in genome synthesis and assembly, particularly in higher organisms, despite facing challenges related to repetitive sequences and cross-species DNA transfer [1][2]. Group 1: Technological Breakthroughs - Tianjin University's team achieved the first precise assembly and cross-species delivery of megabase-scale human DNA, marking a significant milestone in synthetic biology [2]. - The newly developed SynNICE technology enables the precise assembly of highly repetitive human genomic sequences in yeast and facilitates the efficient transfer of synthetic DNA into mouse embryos [3][5]. Group 2: Research Findings - The study successfully demonstrated de novo DNA methylation patterns in mouse embryos, indicating that the synthetic genome can be recognized and modified by the host cell environment [5][7]. - A hierarchical assembly strategy was employed to synthesize a 1.14 Mb region of the human Y chromosome, which is crucial for understanding male infertility [3][7]. Group 3: Implications for Disease Treatment - The research opens new avenues for treating chromosomal abnormalities and could lead to innovative therapeutic strategies for related diseases [7][9]. - The potential for using genetically modified pigs as organ donors for humans could be enhanced, addressing the global organ shortage issue by improving the longevity of transplanted organs [9][10]. Group 4: Future Prospects - The SynNICE technology platform is expected to facilitate large-scale genomic modifications, potentially transforming organ transplantation practices and enhancing the understanding of genetic diseases [11][12]. - The advancements in synthetic genome assembly and transfer techniques are anticipated to propel the field of synthetic biology forward, providing new models for studying epigenetic modifications and chromosomal disorders [10][11].

Core Viewpoint - The article emphasizes the importance of biomanufacturing as a key focus for enhancing economic competitiveness in various countries, including China, which is advancing its manufacturing capabilities through initiatives like green manufacturing and intelligent manufacturing [1]. Group 1: Event Overview - The Fourth Synthetic Biology and Green Biomanufacturing Conference (SynBioCon 2025) will be held from August 20-22 in Ningbo, Zhejiang, focusing on the integration of AI and biomanufacturing, along with four major application areas: green chemicals and new materials, future food, future agriculture, and beauty raw materials [1]. - The conference aims to explore the development trends of the biomanufacturing industry during the 14th Five-Year Plan period and identify innovative technologies and products that will sustain the industry's vitality [1]. Group 2: Organizing Institutions - The conference is organized by Ningbo Detaizhong Research Information Technology Co., Ltd. (DT New Materials) and co-organized by Ningbo Meisai Biological Engineering Co., Ltd. It is supported by various professional committees and alliances in the field of biomanufacturing [2]. Group 3: Highlights of the Conference - The conference will feature forums and closed-door activities to gain insights into the development trends of biomanufacturing, explore new products and technologies, and foster new collaborations [4]. - There will be over 100 financing project roadshows to discover new cooperation opportunities and showcase innovative technological achievements [4]. - Key industry leaders, top universities, capital from parks, and the entire industry chain will gather to discuss advancements and opportunities in biomanufacturing [4]. Group 4: Agenda and Activities - The first day will include a closed-door seminar focusing on the development trends and growth points of biomanufacturing during the 14th Five-Year Plan, inviting 30 industry leaders and experts for in-depth discussions [9]. - The second and third days will feature a macro forum on biomanufacturing, specialized sub-forums on green chemicals, AI in biomanufacturing, future food and agriculture, and beauty raw materials, addressing various key topics and innovations in these fields [13]. - A special session for showcasing scientific achievements and project matchmaking will also be held, inviting 100 innovative projects in the biomanufacturing sector for display and collaboration [14].

Core Insights - The "14th Five-Year Plan" has concluded, and the "15th Five-Year Plan" is being formulated, highlighting a clear and distinct evolution in China's strategic layout in the field of biomanufacturing [1] Group 1: Development Goals - The "15th Five-Year Plan" is a critical period for the comprehensive rise of China's biomanufacturing industry, with a key goal of achieving an added value of over 4 trillion yuan in advanced biomanufacturing and related services, and a biomanufacturing penetration rate exceeding 8% [3] Group 2: Industry Structure - The plan aims to establish three major systems: innovation, industry, and management services, accelerating the development of a "7+1" biomanufacturing industry system, which includes biopharmaceuticals, bio-based chemicals and materials, bioenergy, fermentation, bioengineering food, animal feed, biotechnology equipment, and bio-services [4] Group 3: Future Opportunities - The upcoming SynBioCon 2025 conference will focus on the intersection of AI and biomanufacturing, exploring trends in green chemicals, new materials, future food, future agriculture, and beauty raw materials, while inviting various stakeholders to discuss innovative technologies and products that will sustain the vitality of the biomanufacturing industry [14]